Stacking modular DNA circuitry in cascading self-assembly of spherical nucleic acids
Stacking modular DNA circuitry in cascading self-assembly of spherical nucleic acids
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球形核酸级联自组装中堆叠模块化 DNA 电路
DOI：
10.1039/c7tb01307h
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发表时间：
2017
影响因子：
7
通讯作者：
Liang Haojun
中科院分区：
文献类型：
--
作者：
Yao Dongbao;Xiao Shiyan;Zhou Xiang;Li Hui;Wang Bei;Wei Bing;Liang Haojun
不需要的初始泄漏和渐近泄漏对于制造集成电路至关重要。本文提出了一种结合模拟和实验的新策略，将球形核酸（SNA）的电路扩展到三层。结果表明，基于 SNA 的电路对初始泄漏不敏感，但受渐近泄漏的影响显着，特别是对于两层电路。泄漏对集成系统性能的动态行为和影响可以通过微调立足点域和物质的摩尔比来调节。实验上采用小上游层和大下游层来平衡集成三层电路的稳定性和运行效率。这项工作充分展示了制造模块化级联电路的能力，用于驱动复杂系统中的 SNA 组装，以及调制或编程分子机械以开发自主系统。
Unwanted initial and asymptotic leakages are vital to fabricating integrated circuitries. This paper presents a novel strategy that combines simulations and experiments to extend the circuitries of spherical nucleic acid (SNA) to three layers. The results indicate that SNA-based circuits are insensitive to initial leakage but are significantly influenced by asymptotic leakage, particularly for the two-layer circuit. The dynamic behaviors of and the effects of leakage on the performance of the integrated system can be regulated by fine tuning of toehold domains and the molar ratio of the species. Small upstream layers and a large downstream layer are experimentally adopted to balance the stability and operation efficiency of the integrated three-layer circuitry. This work soundly demonstrates the capability of fabricating modular cascaded circuitry for driving SNA assembly in sophisticated systems, and modulating or programming the molecular machinery for developing an autonomous system.