Physical Analysis of Tissue Mechanics in Amphibian Gastrulation
Physical Analysis of Tissue Mechanics in Amphibian Gastrulation
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两栖动物原肠胚形成组织力学的物理分析
DOI：
10.1093/icb/24.3.657
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发表时间：
1984
影响因子：
2.6
通讯作者：
H. M. Phillips
中科院分区：
文献类型：
--
作者：
H. M. Phillips
在形态发生过程中，细胞间的附着限制了细胞的移动性，因此胚胎组织可能（i）变形为紧密结合的细胞的固体片，（ii）分散为单独的细胞迁移或（iii）作为多细胞液体流动（其中细胞保持聚集，但仍然可以相互滑动）。通过将两栖动物原肠胚形成中的深层胚层模拟为多细胞液体，Davis和我预测并通过实验证实了：（i）体外深层胚层表面张力的面积不变性，（ii）变形深层胚层细胞聚集体中的自发细胞滑移，以及（iii）深层胚层细胞分选和聚集体融合实验中组织表面张力与组织定位的相关性。液体-组织流动涉及内部细胞插入扩张的组织界面（或从收缩的组织界面撤回表面细胞）。组织表面张力是指导这些细胞易位的微观组织特异性粘附差异的宏观反映。这种粘附差异可以独立于或与主动细胞形状变化、趋化性、接触引导和/或趋触性一起在控制胚胎组织重排中起作用。深细胞嵌入在体内发生在整个两栖类原肠胚形成：在外胚层epiboly;在边缘区的扩展和收敛（因此胚孔关闭）;在中胚层退化;可能在前扩展和轴向扩展（因此背收敛）的退化中胚层。组织表面张力的测量可能有助于确定（i）这些细胞嵌入过程中的哪些是主动的，哪些是被动的，（ii）各种微观细胞特性的具体贡献，以调节液体样胚层组装和（iii）在体外和体内控制机制之间的相似性和差异管理两栖动物原肠胚形成。
During morphogenesis, intercellular attachments constrain cell mobility so that embryonic tissues may (i) deform as solid sheets of tightly bound cells, (ii) disperse to migrate as separate cells or (iii) flow as multicellular liquids (in which cells remain aggregated yet can still slide past one another). By modelling deep germ layers as multicellular liquids in amphibian gastrulation, Davis and I have predicted, and then confirmed experimentally, (i) the area-invariance of deep-germ-layer surface tensions in vitro , (ii) spontaneous cell slippage in deformed deep—germ—layer cell aggregates and (iii) correlations of tissue surface tensions with tissue positioning in deep-germ-layer cell-sorting and aggregate—fusion experiments. Liquid—tissue flow involves intercalations of interior cells into expanding tissue interfaces (or withdrawal of surface cells from shrinking tissue interfaces). Tissue surface tensions are macroscopic reflections of the microscopic, tissue—specific adhesive differentials which direct these cell translocations. Such adhesive differentials may act independently of, or together with, active cell—shape changes, chemotaxis, contact guidance and/or haptotaxis in controlling embryonic tissue rearrangements. Deep cell intercalations in vivo occur throughout amphibian gastrulation: during ectodermal epiboly; during marginal—zone extension and convergence (and therefore blastopore closure); during mesodermal involution; and probably during the anterior spreading and axial extension (and therefore dorsal convergence) of involuted mesoderm. Tissue—surface—tension measurements may help determine (i) which of these cell—intercalation processes are active and which are passive, (ii) the specific contributions of various microscopic cell properties to the regulation of liquid—like germ—layer assembly and (iii) similarities and differences between in vitro and in vivo control mechanisms governing amphibian gastrulation.
快速的细胞易位与各种培养细胞与其基质之间形成的紧密接触有关。
影响因子：
4
作者：
Kolega,J;Shure,MS;Chen,WT;Young,ND
通讯作者：
Young,ND