Assessment of Nerve Regeneration Across Nerve Allografts Treated with Tacrolimus
Assessment of Nerve Regeneration Across Nerve Allografts Treated with Tacrolimus
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DOI：
10.1080/10731190802375810
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发表时间：
2008-01-01
期刊：
影响因子：
--
通讯作者：
Li Xin
中科院分区：
文献类型：
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作者：
Han Haisheng;Zuo Songjie;Li Xin
虽然同种异体神经移植的再生是临床上的主要关注点，但很少有文章定量评估动物同种异体神经移植物的长期功能恢复。在这项研究中，功能恢复，组织病理学研究，免疫组织化学变化与FK 506大鼠同种异体神经移植到12周没有屠宰。C57和SD大鼠用于移植。捐献者的神经被切开并移植到接受者体内。坐骨神经用10-0尼龙外膜缝合。共建立了30个移植模型，并分为3组，即FK 506组和非FK 506组。移植神经的功能恢复通过针刺试验、步行轨迹分析和电生理评价进行连续评估。对所有模型进行组织病理学和免疫组化研究。用FK 506处理的同种异体神经移植物在12周内没有免疫排斥反应。同种异体移植物和同种异体移植物的敏感性也有类似的改善。每种移植物均无差异。行走轨迹分析显示神经移植物的运动功能显著恢复。在12周内，每种移植物的组织学结果均无差异。在啮齿动物神经移植模型中，FK 506可防止神经同种异体移植物排斥反应穿过主要组织相容性屏障。感觉恢复似乎比运动功能好上级。FK 506处理的同种异体神经移植物和同种异体神经移植物在功能恢复、组织病理学结果和免疫组化变化方面无显著差异。
Although regeneration of nerve allotransplant is a major concern in the clinic, there have been few papers quantitatively assessing functional recovery of animals' nerve allografts in the long term. In this study, functional recovery, histopathological study, and immunohistochemistry changes of rat nerve allograft with FK506 were investigated up to 12 weeks without slaughtering. C57 and SD rats were used for transplantation. The donor's nerve was sliced and transplanted into the recipient. The sciatic nerve was epineurally sutured with 10-0 nylon. In total, 30 models of transplantation were performed and divided into 3 groups that were either treated with FK506 or not. Functional recovery of the grafted nerve was serially assessed by the pin click test, walking track analysis and electrophysiological evaluations. A histopathological study and immunohistochemistry study were done in the all of the models. Nerve allografts treated with FK506 have no immune rejection through 12 weeks. Sensibility had similarly improved in both isografts and allografts. There has been no difference in each graft. Walk track analysis demonstrates significant recovery of motor function of the nerve graft. No histological results of difference were found up to 12 weeks in each graft. In the rodent nerve graft model, FK506 prevented nerve allograft rejection across a major histocompatibility barrier. Sensory recovery seems to be superior to motor function. Nerve isograft and allograft treated with FK506 have no significant difference in function recovery, histopathological result, and immunohistochemistry changes.