In vivo differentiation of progenitor cells of the periodontal ligament. An experimental study using physical barriers.
In vivo differentiation of progenitor cells of the periodontal ligament. An experimental study using physical barriers.
复制标题
牙周膜祖细胞的体内分化。
DOI：
10.1111/j.1600-051x.1986.tb02244.x
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发表时间：
1986
影响因子：
6.7
通讯作者：
Pettersson,E
中科院分区：
文献类型：
--
作者：
Aukhil,I;Simpson,DM;Suggs,C;Pettersson,E
本研究旨在验证牙骨质再生过程中，牙周膜祖细胞必须与牙根牙本质接触才能分化为成牙骨质细胞的假设。在6只比格犬的下颌骨颊侧皮质板上，采用反折粘骨膜瓣，制作开窗创面。对暴露的牙根表面进行刮治以去除所有牙骨质。一侧暴露的牙根表面用柠檬酸脱矿，而对侧伤口用盐水处理。然后干燥暴露的牙根表面，并将核孔膜（孔径0.1 μ）片附着在暴露的牙根表面的一部分上，以防止祖细胞与牙根牙本质接触。然后用Millipore过滤器覆盖开窗伤口，以促进来自牙周韧带的祖细胞在伤口中的群体。愈合3个月后进行组织学分析，在核孔膜仍然附着在根面牙本质上的标本中，膜上没有看到新的牙骨质。在伤口边缘和核孔膜与牙根牙本质分离的标本中。持续观察到新的结缔组织附着。此外，在酸处理和对照标本中很少观察到牙根吸收。目前的研究结果表明，与牙根牙本质接触可能是祖细胞分化为成牙骨质细胞等形成细胞所必需的。
The present study was designed to test the hypothesis that during regeneration of cementum, the progenitor cells from periodontal ligament must come in contact with root dentin in order to differentiate into cementoblasts. After reflecting mucoperiostea flaps, fenestration wounds were made in the buccal cortical plates of mandibular canines in 6 beagle dogs. The exposed root surfaces were curretted to remove all cementum. The exposed root surface on one side was demineralized with citric acid while the contralateral wound had saline treatment. The exposed root surfaces were then dried and pieces of Nucle‐pore membrane (pore size 0.1 μ) were attached to part of the exposed root surface to prevent contact of progenitor cells with root dentin. The fenestration wounds were then covered with Millipore filter to facilitate the population of wounds by progenitor cells from the periodontal ligament. Histologic analysis was performed after 3 months of healing.In specimens where the Nuclepore membrane had remained attached to root dentin, no new cementum was seen over the membrane. At the borders of the wounds and in specimens where the Nuclepore membrane had detached from root dentin. new connective tissue attachment was consistently seen. Also, root resorption was very rarely observed in both the acid‐treated and control specimens.The present findings suggest that contact with root dentin may be necessary for progenitor cell differentiation into formative cells like cementoblasts.