PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes
PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes
复制标题
DOI：
10.1007/s00436-010-1847-5
复制
发表时间：
2010-06-01
影响因子：
2
通讯作者：
Kapel, Christian M. O.
中科院分区：
文献类型：
--
作者：
Al-Sabi, Mohammad N. S.;Deplazes, Peter;Kapel, Christian M. O.
心血管线虫血管圆线虫在北方欧洲的狐狸和狗群中传播。A.血管痉挛可导致犬严重的临床表现;因此，特异性诊断对于评估其患病率至关重要。本研究采用一种新的针对A.血管。通过筛分对粪便幼虫进行初步分离有利于处理较大的样品体积，并允许从冷冻样品中回收死幼虫。筛选-PCR方法能够识别每2克粪便样品中的单个幼虫，并且不会扩增一系列犬蠕虫的DNA，从而为野生动物监测和犬的确诊提供了一种非侵入性工具。
The cardiovascular nematode Angiostrongylus vasorum is spreading in the fox and dog populations of northern Europe. A. vasorum can result in severe clinical manifestations in dogs; therefore, specific diagnosis is crucial for assessing its prevalence. In the present study, faecal samples from foxes and domestic dogs were tested by a new polymerase chain reaction (PCR) targeting the second internal transcribed region of the ribosomal DNA (ITS2) of A. vasorum. Initial isolation of faecal larvae by sieving facilitated the processing of larger sample volumes and allowed for the recovery of dead larvae from frozen samples. The sieve-PCR method enabled the identification of a single larva per 2 g of faecal sample and did not amplify DNA of a range of canine helminths, thus presenting a non-invasive tool for wildlife surveillance and for confirmative diagnosis in dogs.