Houttuynia cordata water extract suppresses anaphylactic reaction and IgE-mediated allergic response by inhibiting multiple steps of FcεRI signaling in mast cells
Houttuynia cordata water extract suppresses anaphylactic reaction and IgE-mediated allergic response by inhibiting multiple steps of FcεRI signaling in mast cells
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DOI：
10.1016/j.fct.2009.04.025
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发表时间：
2009-07-01
影响因子：
4.3
通讯作者：
Jeong, Hye Gwang
中科院分区：
文献类型：
--
作者：
Han, Eun Hee;Park, Jin Hee;Jeong, Hye Gwang
鱼腥草在韩国被用作传统药物，已知具有抗氧化、抗癌和抗过敏活性。H.然而，cordata仍然未知。在本研究中，我们研究了H. cordato水提取物（HCWE）对小鼠被动皮肤过敏反应（PCA）和大鼠肥大RBL-2 H3细胞IgE介导的过敏反应的影响。口服HCWE可抑制小鼠体内IgE介导的全身PCA。HCWE也减少抗原（DNP-BSA）诱导的释放β-氨基己糖苷酶，组胺，和活性氧在IgE致敏的RBL-2 H3细胞。此外，HCWE抑制IgE致敏的RBL-2 H3细胞中抗原诱导的IL-4和TNF-α的产生和表达。HCWE抑制抗原诱导的NF-κ B活化和I κ B-α降解。为了研究HCWE对脱粒和细胞因子产生的抑制机制，我们检测了细胞内Fc β RI信号分子的激活。HCWE抑制抗原诱导的Syk、林恩、LAT、Gab 2和PLC γ 2的磷酸化。再往下游。抗原诱导的Akt和MAP激酶（ERK 1/2和JNK 1/2，但不是p38 MAP激酶）的磷酸化被HCWE抑制。综上所述，HCWE的体内/体外抗过敏作用表明，该药剂通过抑制肥大细胞中的细胞因子和Fc β RI依赖性信号级联的多个事件在炎性过敏性疾病中可能具有治疗应用。(C)2009爱思唯尔有限公司保留所有权利。
Houttuynia cordata has been used as a traditional medicine in Korea and is known to have antioxidant, anti-cancer and anti-allergic activities. The precise effect of H. cordata, however, remains unknown. in this study, we investigated the effects of H. cordato water extract (HCWE) on passive cutaneous anaphylaxis (PCA) in mice and on IgE-mediated allergic response in rat mast RBL-2H3 cells. Oral administration of HCWE inhibited IgE-mediated systemic PCA in mice. HCWE also reduced antigen (DNP-BSA)-induced release of beta-hexosaminidase, histamine, and reactive oxygen species in IgE-sensitized RBL-2H3 cells. In addition, HCWE inhibited antigen-induced IL-4 and TNF-alpha production and expression in IgE-sensitized RBL-2H3 cells. HCWE inhibited antigen-induced activation of NF-kappa B and degradation of I kappa B-alpha. To investigate the inhibitory mechanism of HCWE on degranulation and cytokine production, we examined the activation of intracellular Fc epsilon RI signaling molecules. HCWE suppressed antigen-induced phosphorylation of Syk, Lyn, LAT, Gab2, and PLC gamma 2. Further downstream. antigen-induced phosphorylation of Akt and MAP kinases (ERK1/2 and JNK1/2 but not p38 MAP kinase) were inhibited by HCWE. Taken together, the in vivo/in vitro anti-allergic effect of HCWE suggests possible therapeutic applications of this agent in inflammatory allergic diseases through inhibition of cytokines and multiple events of Fc epsilon RI-dependent signaling cascades in mast cells. (C) 2009 Elsevier Ltd. All rights reserved.