Cytokinin-induced gene expression in cultured green cells of Nicotiana tabacum identified by fluorescent differential display
Cytokinin-induced gene expression in cultured green cells of Nicotiana tabacum identified by fluorescent differential display
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DOI：
10.1271/bbb.65.1275
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发表时间：
2001-06-01
影响因子：
1.6
通讯作者：
Yoshida, S
中科院分区：
文献类型：
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作者：
Kimura, T;Nakano, T;Yoshida, S
植物激素细胞分裂素能维持培养的绿色烟草细胞的生长和质体发育。继代培养到无细胞分裂素培养基后，当细胞分裂素处理恢复时，分析细胞分裂素诱导的生理变化。在细胞分裂素诱导后1周观察到叶绿素生物合成和光合基因表达的变化，在细胞分裂素诱导后2周观察到细胞生长的变化。利用荧光差异显示技术从这些细胞中分离到两个细胞分裂素诱导基因（cig）。北方分析证实，这些cig的表达诱导的天然和合成的细胞分裂素。cig 1的表达也受到脱落酸的诱导，其cDNA序列与脯氨酸脱氢酶基因相似。cig 2的表达是细胞分裂素特异性的，不受其他植物激素的诱导。cig 2编码的氨基酸序列与调节翻译起始的GDP/GTP交换因子eIF 2B相似。这些cig的表达表明一个复杂的诱导系统，涉及细胞分裂素和其他植物激素。
The cell growth and plastid development of cultured green tobacco cells were maintained by the phytohormone cytokinin. After subculture into cytokinin-free medium, when cytokinin treatment was resumed, physiological changes induced by cytokinin were analyzed. Changes in chlorophyll biosynthesis and photosynthetic gene expression were observed 1 week after cytokinin induction, and changes in cell growth were observed 2 weeks after cytokinin induction. Two cytokinin-induced genes (cig) were isolated from these cells using the fluorescent differential display technique. Northern analysis confirmed that expression of these cig was induced by both natural and synthetic cytokinins. The expression of cig1 was also induced by abscisic acid, and its cDNA sequence was similar to the proline dehydrogenase gene. The expression of cig2 is specific to cytokinin and is not induced by other phytohormones, The amino acid sequence encoded by cig2 is similar to the GDP/GTP exchange factor eIF2B, which regulates translation initiation. The expression of these cig suggests a complex induction system involving cytokinin and other phytohormones.