Effects of a mesoporous bioactive glass on osteoblasts, osteoclasts and macrophages
Effects of a mesoporous bioactive glass on osteoblasts, osteoclasts and macrophages
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DOI：
10.1016/j.jcis.2018.05.099
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发表时间：
2018-10-15
影响因子：
9.9
通讯作者：
Portoles, M. T.
中科院分区：
文献类型：
--
作者：
Gomez-Cerezo, N.;Casarrubios, L.;Portoles, M. T.
摩尔组成为 75SiO(2)-20CaO-5P(2)O(5) (MBG-75S) 的介孔生物活性玻璃 (MBG) 已被合成为用于骨再生目的的潜在生物陶瓷。 X射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）、氮吸附研究和透射电子显微镜（TEM）表明MBG-75S具有高度有序的介孔结构，具有高表面积和孔隙率，这可以解释与周围介质的高离子交换率（主要是钙和硅可溶物质）。 MBG-75S 在与 Saos-2 成骨细胞样细胞接触时表现出高度的生物相容性。浓度高达 1 mg/ml 不会导致形态或细胞周期的显着改变。关于对破骨细胞的影响，MBG-75S 允许 RAW-264.7 巨噬细胞分化为破骨细胞样细胞，但表现出吸收活性降低。这些结果表明MBG-75S不抑制破骨细胞生成，但降低破骨细胞骨吸收能力。最后，体外研究重点关注先天免疫反应，证明 MBG-75S 允许巨噬细胞增殖，而不诱导其极化为 M1 促炎表型。这种体外行为表明 MBG-75S 在体内条件下只会诱导所需的先天免疫反应，而不会出现进一步的炎症并发症。成骨细胞、破骨细胞和巨噬细胞的整体行为使该 MBG 成为骨质疏松患者骨移植应用中非常有趣的候选者。 (C) 2018 Elsevier Inc. 保留所有权利。
A mesoporous bioactive glass (MBG) of molar composition 75SiO(2)-20CaO-5P(2)O(5) (MBG-75S) has been synthetized as a potential bioceramic for bone regeneration purposes. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), nitrogen adsorption studies and transmission electron microscopy (TEM) demonstrated that MBG-75S possess a highly ordered mesoporous structure with high surface area and porosity, which would explain the high ionic exchange rate (mainly calcium and silicon soluble species) with the surrounded media. MBG-75S showed high biocompatibility in contact with Saos-2 osteoblast-like cells. Concentrations up to 1 mg/ml did not lead to significant alterations on either morphology or cell cycle. Regarding the effects on osteoclasts, MBG-75S allowed the differentiation of RAW-264.7 macrophages into osteoclast-like cells but exhibiting a decreased resorptive activity. These results point out that MBG-75S does not inhibit osteoclastogenesis but reduces the osteoclast boneresorbing capability. Finally, in vitro studies focused on the innate immune response, evidenced that MBG-75S allows the proliferation of macrophages without inducing their polarization towards the M1 pro-inflammatory phenotype. This in vitro behavior is indicative that MBG-75S would just induce the required innate immune response without further inflammatory complications under in vivo conditions. The overall behavior respect to osteoblasts, osteoclasts and macrophages, makes this MBG a very interesting candidate for bone grafting applications in osteoporotic patients. (C) 2018 Elsevier Inc. All rights reserved.