PHAGOCYTOSIS AND KILLING OF COMMON BACTERIAL PATHOGENS OF THE LUNG BY HUMAN ALVEOLAR MACROPHAGES
PHAGOCYTOSIS AND KILLING OF COMMON BACTERIAL PATHOGENS OF THE LUNG BY HUMAN ALVEOLAR MACROPHAGES
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DOI：
10.1093/infdis/152.1.4
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发表时间：
1985-01-01
影响因子：
6.4
通讯作者：
LAWRENCE, EC
中科院分区：
文献类型：
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作者：
JONSSON, S;MUSHER, DM;LAWRENCE, EC
探讨决定肺部对常见呼吸道病原体感染易感性的因素，以及对非典型流感嗜血杆菌的吞噬和杀灭作用。流感B型;肺炎链球菌III、VI和XIV型; S.肺炎III型;和金黄色葡萄球菌科万I的研究。在用10%合并的人血清调理后，平均摄取（±）。SD）的非典型H.流感病毒（67.5 ± 0.01）。15.0%）、未包封S.肺炎III型（71.2 ±. 4.8%）和S.金黄色葡萄球菌（79.1 . ±. 10.2%）显著高于H.流感病毒B型（40.1 . ±. 15.0%）和S.肺炎III型（4.4 . ±. 3.1%）、VI（11.8 ±. 9.6%）或XIV（8.7 . ±. 7.0%）。不可分型H.在调理作用后，摄入流感病毒的人血清比摄入H.流感病毒B型，以及包裹的S. pneumoniae的表达没有被多达80%的合并的人血清增强。未包囊S. pneumoniae III型和非分型H.流感嗜血杆菌是快速和完整的，并对应于吞噬的程度，但尽管高摄取，S。金黄色葡萄球菌被缓慢而不完全地杀死。对S.金黄色葡萄球菌被缓慢而不完全地杀死。对S.肺炎链球菌和H.因此，作为肺病原体的流感是由包囊和正常人血清的不充分调理作用共同决定的，而S.金黄色葡萄球菌可能与机体对肺泡巨噬细胞胞内杀伤的相对抵抗力有关。
To investigate factors that determine susceptibility of the lungs to infection with common respiratory pathogens, phagocytosis and killing of nontypable Haemophilus influenzae; H. influenzae type b; Streptococcus pneumoniae types III, VI, and XIV; an unencapsulated variant of S. pneumoniae type III; and Staphylococcus aureus Cowan I, by using human alveolar macrophages obtained by bronchoalveolar lavage of healthy nonsmokers, was studied. After opsonization with 10% pooled human serum, mean uptake (.+-. SD) of nontypable H. influenzae (67.5 .+-. 15.0%), unencapsulated S. pneumoniae type III (71.2 .+-. 4.8%) and S. aureus (79.1 .+-. 10.2%) was significantly greater (P < 0.01) than that of H. influenzae type b (40.1 .+-. 15.0%), and S. pneumoniae types III (4.4 .+-. 3.1%), VI (11.8 .+-. 9.6%) or XIV (8.7 .+-. 7.0%). Nontypable H. influenzae was ingested after opsonization with much less pooled human serum than was H. influenzae type b, and uptake of encapsulated S. pneumoniae was not enhanced by as much as 80% pooled human serum. Intracellular killing of unencapsulated S. pneumoniae type III and nontypable H. influenzae was rapid and complete and corresponded to the degree of phagocytosis, but despite a high uptake, S. aureus were killed slowly and incompletely. The virulence of S. aureus were killed slowly and incompletely. The virulence of S. pneumoniae and H. influenze as lung pathogens is thus determined jointly by encapsulation and the inadequate opsonizing effect of normal human serum, whereas that of S. aureus may be related to the organism''s relative resistance to intracellular killing by alveolar macrophages.