The enzymic formation of methylenedioxyphenyl derivative exhibiting an isocyanide-like spectrum with reduced cytochrome P-450 in hepatic microsomes.
The enzymic formation of methylenedioxyphenyl derivative exhibiting an isocyanide-like spectrum with reduced cytochrome P-450 in hepatic microsomes.
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DOI：
10.3109/00498257109112269
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发表时间：
1971
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影响因子：
--
通讯作者：
Michael R. Franklin
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文献类型：
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作者：
Michael R. Franklin
摘要 1．亚甲基二氧基苯基化合物（胡椒基丁醚、亚甲基二氧基苯、异黄樟素）在氧和NADPH（但不包括NADH）存在下被大鼠肝微粒体代谢，产生具有类似异氰化物吸收光谱的产物，并还原了肝微粒体的细胞色素P-450。 2．六巴比妥的存在抑制了类异氰化物谱的形成，可能是通过抑制代谢物的形成来实现的。3．代谢物与还原型细胞色素P-450的复合物的光谱吸收特性随pH的变化而变化。4。代谢物或形成的代谢物-细胞色素 P-450 复合物足够稳定，能够在微粒体细胞色素 P-450 的氧化和再还原中存活。
Abstract1. Methylenedioxyphenyl compounds (piperonyl butoxide, methylene-dioxybenzene, isosafrole) were metabolized by rat liver microsomes in the presence of oxygen and NADPH (but not NADH) to give products which exhibited an isocyanide-like absorption spectrum with reduced cytochrome P-450 of liver microsomes.2. The presence of hexobarbital inhibited the formation of the isocyanide-like spectrum, presumably by inhibiting the formation of the metabolite.3. The optical spectrum absorption characteristics of the complex of the metabolite with reduced cytochrome P-450 varied with changes in pH.4. The metabolite, or the metabolite-cytochrome P-450 complex formed, was sufficiently stable to survive the oxidation and re-reduction of the microsomal cytochrome P-450.