Comparative study in vivo and in vitro of the differentiation of immunoreactive corticotropic cells in fetal rat anterior pituitary.
Comparative study in vivo and in vitro of the differentiation of immunoreactive corticotropic cells in fetal rat anterior pituitary.
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胎鼠垂体前叶免疫反应性促肾上腺皮质细胞分化的体内和体外比较研究。
DOI：
10.1159/000123391
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发表时间：
1982
影响因子：
4.1
通讯作者：
P. Dubois
中科院分区：
文献类型：
--
作者：
M. Bégeot;M. Dubois;P. Dubois
为了研究促肾上腺皮质细胞分化的机制，我们在体内和体外用抗β -(1-24)和α -(17-39) ACTH和β - lph、α -和β -内啡肽的抗血清对胎鼠垂体前叶进行了免疫细胞学研究。在体内，这些细胞在妊娠16天出现，两种免疫反应的出现时间没有任何差异。用含胎牛血清的培养基或含胰岛素和转铁蛋白的培养基代替胎牛血清，从妊娠12 ~ 15天外植的腺垂体原基在器官培养中维持到21天，也检测到相同的免疫反应性。这些免疫反应性细胞首先在不同的实验原基中被检测到，经过一段最短的培养时间，相当于在体内的最后16天。然而，无论培养时间长短，免疫反应细胞的平均细胞质面积与外植时间有关。这些数据表明：(1)本研究中使用的培养基性质对促皮质细胞的分化没有影响；(2)这种细胞类型似乎过早地（在第12天之前）被一种或几种来源不明的物质所控制；(3)正常发育似乎需要（在第14天之前）存在一些性质和起源尚待阐明的因素。
In order to study the mechanisms of the differentiation of adenohypophysial corticotropic cells, an immuno-cytological study was performed in fetal rat anterior pituitary in vivo and in vitro with antisera against beta-(1-24) and alpha-(17-39) ACTH and beta-LPH, alpha- and beta-endorphins. In vivo, these cells appeared at 16 days of gestation without any difference in the timing of appearance of the two immunoreactivities. The same immunoreactivity was also detected in adenohypophysial primordia explanted from 12 to 15 days of gestation and maintained in organ culture until 21 days by using either medium containing fetal calf serum or medium containing insulin and transferrin instead of fetal calf serum. These immunoreactive cells were first detected in the different experimental primordia after a minimal period of culture, corresponding to a final equivalent of 16 days as in vivo. However, the mean cytoplasmic area of immunoreactive cells increased in relation to the day of explantation whatever the duration of culture. These data suggest: (1) the nature of culture medium used in this study has no influence on the differentiation of the corticotropic cells; (2) this cell type seems to be committed precociously (before day 12) by one or several substances of unknown origin; (3) the normal development seems to require the presence of factors (before day 14) whose nature and origin remain to be elucidated.