Characterization of class I Newcastle disease virus isolates from Hong kong live bird markets and detection using real-time reverse transcription-PCR
Characterization of class I Newcastle disease virus isolates from Hong kong live bird markets and detection using real-time reverse transcription-PCR
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DOI：
10.1128/jcm.02594-06
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发表时间：
2007-04-01
影响因子：
9.4
通讯作者：
Afonso, Claudio L.
中科院分区：
文献类型：
--
作者：
Kim, L. Mia;King, Daniel J.;Afonso, Claudio L.
采用美国食品药品管理局（usda）验证的基质基因实时逆转录pcr （RT-PCR）检测方法，未检测到从香港活禽市场（LBMs）分离的新城疫病毒。根据融合基因的系统发育分析，这些病毒与在美国lbm和野生水禽中发现的透镜性I类病毒有关。一种替代的实时RT-PCR测定，补充了基质基因测定，以有效地检测I类病毒。
Newcastle disease viruses isolated from Hong Kong live bird markets (LBMs) were not detected by a USDA-validated matrix gene real-time reverse transcription-PCR (RT-PCR) assay. Based upon phylogenetic analysis of the fusion gene, these viruses were related to lentogenic class I viruses found in U.S. LBMs and wild waterfowl. An alternative real-time RT-PCR assay which complements the matrix gene assay was developed to efficiently detect class I viruses.