Response of two mouse tumours to hyperthermia with CCNU or melphalan.
Response of two mouse tumours to hyperthermia with CCNU or melphalan.
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DOI：
10.1038/bjc.1982.3
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发表时间：
1982-01
影响因子：
8.8
通讯作者：
Stephens, T C
中科院分区：
文献类型：
--
作者：
Joiner, M C;Steel, G G;Stephens, T C
本文研究了B16黑色素瘤和刘易斯肺癌对热疗联合不同剂量的环己亚硝脲或美法仑的体内反应。为了获得剂量-反应曲线和不同处理的定量比较，使用琼脂集落测定来测量来自切除的肿瘤的细胞的存活。对于加热实验，每只动物使用2个肿瘤，一个加热，一个不加热，允许所有其他因素保持不变。当肿瘤在43 ℃水浴中浸泡1小时时，从剂量-反应曲线的斜率测量的热增强比（TER）对于CCNU高达1.6，对于美法仑高达2.4。在43 ℃下1小时，可观察到约10年的直接热杀死。麻醉剂Saffan也增强药物细胞杀伤;在刘易斯肺肿瘤中测量到美法仑的最大剂量调节因子（2.7）。加热的持续时间和水浴温度都影响CCNU对细胞杀伤的增强，治疗后0至3 1/2小时之间的肿瘤切除时间也是如此。3 1/2和24 h的效果无差异。热与CCNU之间的相互作用随热与CCNU之间的时间间隔的变化而变化。结果表明，热药并用效果最好。
The in vivo response of B16 melanoma and Lewis lung carcinoma to combinations of hyperthermia and graded doses of CCNU or Melphalan was studied. To obtain dose-response curves and quantitative comparisons of different treatments, an agar-colony assay was used to measure survival of cells from excised tumours. For heating experiments, the use of 2 tumours per animal, one heated and one not, allowed all other factors to be kept constant. When tumours were immersed in a water-bath at 43 degrees C for 1 h, Thermal Enhancement Ratios (TER) measured from the slopes of the dose-response curves were up to 1.6 for CCNU and 2.4 for Melphalan. Direct heat killing of about 1 decade was seen for 1 h at 43 degrees C. The anaesthetic Saffan also enhanced drug cell kill; the largest Dose Modifying Factor (2.7) was measured for Melphalan in the Lewis lung tumour. The duration of heating, and waterbath temperature, both influenced the enhancement of cell killing by CCNU, as did the time of excision of tumours between 0 and 3 1/2 h after treatment. There was no difference in effect between 3 1/2 and 24 h. The interaction between heat and CCNU varied if the interval between them was altered. The maximum effect was found if the heat and drug were given in close sequence.