Retinal development and the expression profiles of opsin genes during larval development in Takifugu rubripes
Retinal development and the expression profiles of opsin genes during larval development in Takifugu rubripes
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红鳍东方鲀幼虫发育过程中的视网膜发育和视蛋白基因的表达谱
DOI：
10.1111/jfb.15270
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发表时间：
2022
影响因子：
2
通讯作者：
Hongwei Yan
中科院分区：
文献类型：
--
作者：
Qi Zhang;Yumeng Wu;Weiyuan Li;Jia Wang;Huiting Zhou;Lei Zhang;Qi Liu;Liu Ying;Hongwei Yan
鱼苗的感光能力取决于视网膜的结构以及视网膜和非视网膜光感受器中表达的视蛋白。本研究对红东方鲀（Takifugu rubripes）幼鱼早期发育过程中视网膜的结构和视蛋白基因的表达进行了研究。组织学观察表明，出膜后1天，视网膜已分为7层。视网膜色素上皮层（retinal pigment epithelial layer，RPE）、光感受器层（photoreceptor layer，PRos/is）、外核层（outer nuclear layer，ONL）、外丛状层（outer plexiform layer，OPL）、内核层（inner nuclear layer，INL）、内丛状层（inner plexiform layer，IPL）和神经节细胞层（ganglion cell layer，GCL）。2dah时，可见视网膜内的8层结构，即视神经纤维层（optic fiber layer，OFL）。通过测量各层的厚度，发现ONL、OPL、INL、IPL、GCL和OFL的厚度呈相反的发展趋势。同时测定了ONL、INL和GCL的核密度以及ONL/INL、ONL/GCL和INL/GCL的比值，ONL/GCL的比值在2d的1.9 ~ 8d的3.4之间，不同发育阶段之间差异不显著（P> 0.05）。INL/GCL比值在2 ~ 18日龄之间差异不显著（P> 0.05），为1.2 ~ 2.0。真实的实时定量PCR结果显示，从1d开始，RH 1、LWS、RH 2 - 1、RH 2 - 2、SWS 2、视杆细胞视蛋白、视蛋白3和视蛋白5的表达就可以检测到。这些结果表明，发育良好的视网膜和视蛋白的早期表达的T。在从内源性到混合喂养的过渡时期，红血丝可能对孵化后早期生命阶段基于视觉的生存技能至关重要。
The light‐sensitive capacity of fish larvae is determined by the structure of the retina and the opsins expressed in the retinal and nonretinal photoreceptors. In this study, the retinal structure and expression of opsin genes during the early developmental stage ofTakifugu rubripeslarvae were investigated. Histological examination showed that at 1 days after hatching (dah), seven layers were observed in the retina ofT. rubripeslarva, including the pigment epithelial layer [retinal pigment epithelium layer (RPE)], photoreceptor layer (PRos/is), outer nuclear layer (ONL), outer plexiform layer (OPL), inner nuclear layer (INL), inner plexiform layer (IPL) and ganglion cell layer (GCL). At 2 dah, optic fibre layer (OFL) can be observed, and all eight layers were visible in the retina. By measuring the thickness of each layer, opposing developmental trends were found in the thickness of ONL, OPL, INL, IPL, GCL and OFL. The nuclear density of ONL, INL and GCL and the ratios of ONL/INL, ONL/GCL and INL/GCL were also measured and the ratio of ONL/GCL ranged from 1.9 at 2 dah to 3.4 at 8 dah and no significant difference was observed between the different developmental stages (P> 0.05). No significant difference was observed for the INL/GCL ratio between the different developmental stages, which ranged from 1.2 at 2 dah to 2.0 at 18 dah (P> 0.05). The results of quantitative real‐time polymerase chain reaction (PCR) showed that the expression ofRH1,LWS,RH2‐1,RH2‐2,SWS2,rod opsin,opsin3andopsin5could be detected from 1 dah. These results suggest that the well‐developed retina and early expression of the opsins ofT. rubripesduring the period of transition from endogenous to mixed feeding might be critical for vision‐based survival skills during the early life stages after hatching.