Hatching out goldfish from common carp eggs:: interspecific androgenesis between two cyprinid species
Hatching out goldfish from common carp eggs:: interspecific androgenesis between two cyprinid species
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DOI：
10.1139/gen-41-4-573
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发表时间：
1998-08-01
期刊：
影响因子：
3.1
通讯作者：
Horváth, L
中科院分区：
文献类型：
--
作者：
Bercsényi, M;Magyary, I;Horváth, L
我们已经成功地在两个鲤科物种之间进行了种间雄核发育。用3个不同金鱼品种的新鲜精子和冷冻精子受精于辐照鲤鱼卵，然后对单倍体胚胎进行热休克以恢复二倍体并产生可存活的后代。来自十几个不同实验的雄性二倍体金鱼后代被多次筛选出四个表型标记。在雄核发育的金鱼后代中只发现了金鱼特有的颜色和其他表型标记；在目测的1500多个个体中没有检测到来自普通鲤鱼的标记。用随机扩增多态性DNA技术在基因组水平上对亲本和后代进行比较。雄核发育金鱼的随机扩增多态性DNA图谱仅含有父本带，从而证实了表型分析的结果。据我们所知，这是第一次成功地对两个不同物种进行种间雄激素发育，从而产生可存活的后代。
We have successfully performed interspecific androgenesis between two cyprinid species. Gamma-ray irradiated eggs of common carp were fertilized with fresh and cryopreserved sperm of three different goldfish varieties and the haploid embryos were then heat-shocked to restore diploidy and to produce viable offspring. Androgenic diploid goldfish progenies from over a dozen different experiments were screened for four phenotypic markers several times. Color and other phenotypic markers characteristic of goldfish were found exclusively among androgenetic goidfish progenies; no markers originating from common carp were detected in over 1500 individuals investigated visually. RAPD assay was used to compare the parents and the offspring at the genomic level. The RAPD pattern of the androgenetic goldfish contained exclusively paternal bands, thereby confirming the results of the phenotypic analysis. According to our knowledge, this is the first successful interspecific androgenesis performed with two different species resulting in viable offspring.