Determination of progesterone based on the enhancement effect of surfactants in linear sweep polarography
Determination of progesterone based on the enhancement effect of surfactants in linear sweep polarography
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DOI：
10.1016/s0003-2670(00)80874-0
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发表时间：
1991-07
影响因子：
6.2
通讯作者：
Shengshui Hu;Yi-chen Yan;Zaofan Zhao
中科院分区：
文献类型：
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作者：
Shengshui Hu;Yi-chen Yan;Zaofan Zhao
本文提出了一种快速、灵敏测定孕酮的线性扫描极谱法。在碱性介质（0.01 M NaOH，pH 9）中，当样品溶液中加入低浓度的阳离子表面活性剂如十六烷基三甲基溴化铵（CTAB）时，孕酮可在滴汞电极上被还原，峰电流大大增加。由于黄体酮和十六烷基三甲基溴化铵在电极表面的协同吸附，极谱波的灵敏度比没有十六烷基三甲基溴化铵时高40倍。获得了2 × 10− 8 M的低检测限。峰电流与孕酮浓度在8 × 10 - 6 - 4 × 10 - 8 M范围内呈线性关系。研究了孕酮的电化学行为，证实了其存在吸附还原波。该方法用于测定药物制剂和孕妇血液中的孕酮。
A rapid and sensitive linear sweep polarographic method for the determination of progesterone is described. In basic media (0.01 M borax, pH 9), progesterone can be reduced at a dropping mercury electrode and the peak current increases greatly when low concentrations of cationic surfactants such as cetyltrimethylammonium bromide (CTAB) are added to the sample solution. Because of synergistic adsorption of progesterone and CTAB on the electrode surface, the polarographic wave exhibits a sensitivity that is 40 times greater than that in the absence of CTAB. A low detection limit of 2 × 10−8M is obtained. A linear relationship between peak current and concentration of progesterone holds in the range 8 × 10−6−4 × 10−8M. The electrochemical behaviour of progesterone was investigated, and an adsorptive reduction wave was confirmed. The method was developed for the assay of progesterone in pharmaceutical preparations and in the blood of pregnant women.