d-Amino Acid Oxidase Immobilized on Pt Nanoparticle-Loaded Porous SiO2 Nanospheres Coated with a Zirconium-Based Coordination Polymer for Catalytic Deamination of d-Alanine
d-Amino Acid Oxidase Immobilized on Pt Nanoparticle-Loaded Porous SiO2 Nanospheres Coated with a Zirconium-Based Coordination Polymer for Catalytic Deamination of d-Alanine
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DOI：
10.1021/acsanm.1c02825
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发表时间：
2021-10
影响因子：
5.9
通讯作者：
Y. Bu;Lingyan Hu;Wei Feng
中科院分区：
文献类型：
--
作者：
Y. Bu;Lingyan Hu;Wei Feng
Porous SiO2nanospheres are prepared and loaded with platinum (Pt) nanoparticles (Pt&SiO2). The Pt&SiO2nanospheres are coated with a zirconium-based coordination polymer (Pt&SiO2&Zr-cp). Etching of the core SiO2with NaOH produces hollow nanospheres Pt&h-SiO2&Zr-cp.d-Amino acid oxidase (DAAO) with hexahistidine tags was absorbed onto the hollow nanospheres strongly, by utilizing the coordinative interactions between Zr(IV) and the hexahistidine tags. The conjugate DAAO&Pt&h-SiO2&Zr-cp possesses two-enzyme catalysis capability. The adsorbed DAAO catalyzes oxidative deamination ofd-amino acids, and hydrogen peroxide (H2O2) is evolved as a byproduct. The loaded Pt nanoparticles possess peroxidase activity, decomposing H2O2into H2O and O2. Thus, the byproduct inhibitory effect can be minimized. In addition, the generated dioxygen O2can be used to oxidize the reduced cofactor FDA of the adsorbed DAAO. The hybrid DAAO&Pt&h-SiO2&Zr-cp was applied for catalytic deamination ofd-alanine and exhibited a catalytic efficiency more than four times that of free DAAO.
