Determining the absolute surface hydrophobicity of microparticulates using thin layer wicking
Determining the absolute surface hydrophobicity of microparticulates using thin layer wicking
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DOI：
10.1016/s0168-3659(98)00191-6
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发表时间：
1999-05-20
影响因子：
10.8
通讯作者：
Sinko, PJ
中科院分区：
文献类型：
--
作者：
Norris, DA;Puri, N;Sinko, PJ
表面疏水性是微粒（MP）穿过生物屏障运输的重要因素。我们之前在实验室中证明，聚苯乙烯 MP 的表面特性会影响胃肠道 (GI) 粘液和粘蛋白的扩散和运输。不幸的是，目前用于评估 MP 表面疏水性的大多数方法都涉及相对测量，从而产生排名顺序而不是绝对疏水性值。为了获得跨实验室、聚合物、制造方法等有意义的比较和相关性，有必要对疏水性进行绝对评估。开发并验证了改进的薄层芯吸 (TLW) 技术，可以确定完整 MP 的绝对表面疏水性。通过构建标准曲线并将通过在显微镜载玻片上干燥的 MP 的溶剂上升速率与 PLGA 聚合物的已知接触角进行比较来验证 TLW 方法。使用 TLW 技术评估表面接触角范围在 67.04 度至 90.18 度之间的 MP。改进的 TLW 技术还使用表面改性聚苯乙烯和 OVA MP 成功进行了验证。根据当前研究的结果，改进的 TLW 技术似乎是评估完整 MP 表面疏水性的可靠定量方法。 (C) 1999 Elsevier Science B.V. 保留所有权利。
Surface hydrophobicity is an important factor in the transport of microparticulates (MPs) across biological barriers. We have previously shown in our laboratory that the surface properties of polystyrene MPs influence the diffusion and transport through gastrointestinal (GI) mucus and mucin. Unfortunately, most currently used methods for evaluating the surface hydrophobicity of MPs involve a relative measurement resulting in a rank order rather than an absolute hydrophobicity value. Obtaining an absolute assessment of hydrophobicity is necessary in order to obtain meaningful comparisons and correlations across laboratories, polymers, methods of fabrication, and so on. A modified thin layer wicking (TLW) technique was developed and validated to allow for the determination of absolute surface hydrophobicity of intact MPs. The TLW method was validated by constructing a standard curve and comparing the rate of solvent rise through MPs dried on microscope slides to the known contact angle of PLGA polymers. MPs with surface contact angles ranging between 67.04 degrees and 90.18 degrees were evaluated using the TLW technique. The modified TLW technique was also successfully Validated using surface-modified polystyrene and OVA MPs. Based on the results of the current study, the modified TLW technique appears to be a reliable and quantitative method for assessing the surface hydrophobicity of intact MPs. (C) 1999 Elsevier Science B.V. All rights reserved.