Comparative study of the effects of cigarette smoke and electronic cigarettes on human gingival fibroblast proliferation, migration and apoptosis
Comparative study of the effects of cigarette smoke and electronic cigarettes on human gingival fibroblast proliferation, migration and apoptosis
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DOI：
10.1016/j.fct.2018.05.049
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发表时间：
2018-08-01
影响因子：
4.3
通讯作者：
Rouabhia, Mahmoud
中科院分区：
文献类型：
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作者：
Alanazi, Humidah;Park, Hyun Jin;Rouabhia, Mahmoud
为了减少与吸烟有关的疾病，人们提出了电子烟等替代产品。然而，尽管电子烟越来越受欢迎，但其潜在的毒性在很大程度上仍然未知。在这项研究中，人牙龈成纤维细胞反复暴露于香烟烟雾冷凝物（CSC）和富含尼古丁（NR）或无尼古丁（NF）的电子蒸汽冷凝物，每天一次，持续60分钟，持续不同的时间段。然后，它们被用于进行不同的分析。结果表明，细胞暴露于CSC或NR缩合物显示出改变的形态和降低的增殖率，如通过MTT和BrdU测定所确定的。与对照中记录的相比，暴露于CSC或电子蒸汽冷凝物的成纤维细胞培养物也显示出增加的TUNEL阳性凋亡细胞水平。此外，细胞划痕试验表明，反复暴露于CSC或电子蒸汽冷凝物延迟了成纤维细胞迁移和伤口愈合。应该注意的是，CSC比NR和NF电子蒸汽冷凝物对牙龈成纤维细胞的损害更大。因此，代表性的损害链转化为CSC > NR电子蒸汽冷凝物> NF电子蒸汽冷凝物。
In an effort to reduce smoking-related diseases, alternative products such as e-cigarettes have been proposed. However, despite their growing popularity, the potential toxicity of e-cigarettes remains largely unknown. In this study, human gingival fibroblasts were repeatedly exposed to cigarette smoke condensate (CSC) and to nicotine-rich (NR) or nicotine-free (NF) e-vapor condensates for 60 min once a day for various time periods. They were then used to perform different analyses. Results indicate that cells exposed to CSC or NR condensates showed an altered morphology and a reduced proliferation rate, as ascertained by MTT and BrdU assays. Fibroblast cultures exposed to either CSC or e-vapor condensates also showed increased levels of TUNEL-positive apoptotic cells, compared to that recorded in the control. Furthermore, the cell scratch test revealed that repeated exposures to CSC or to e-vapor condensates delayed both fibroblast migration and wound healing. It should be noted that CSC was much more damageable to gingival fibroblasts than were the NR and NF e-vapor condensates. The representative chain of damage thus translates to CSC > NR e-vapor condensate > NF e-vapor condensate.