Correlation between Helicobacter pylori OipA protein expression an oipA gene switch status
Correlation between Helicobacter pylori OipA protein expression an oipA gene switch status
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DOI：
10.1128/jcm.42.5.2279-2281.2004
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发表时间：
2004-05-01
影响因子：
9.4
通讯作者：
Yamaoka, Y
中科院分区：
文献类型：
--
作者：
Kudo, T;Nurgalieva, ZZ;Yamaoka, Y
合成OipA肽或重组OipA蛋白的多克隆抗血清检测幽门螺杆菌中的OipA表达，并与通过PCR序列确定的功能性OipA状态相关（灵敏度和特异性>94%）。免疫印迹法是检测重要毒力因子OipA表达的一种简便、准确的方法。
Polyclonal antisera to either a synthetic OipA peptide or a recombinant OipA protein detected OipA expression in Helicobacter pylori and correlated with functional oipA status determined by PCR sequence (sensitivity and specificity of >94%). Immunoblotting is a simple and accurate method for detecting expression of the important virulence factor OipA.