Sequence of the S10 cDNA from 'McIntosh' Apple and a PCR-digestion Identification Method
Sequence of the S10 cDNA from 'McIntosh' Apple and a PCR-digestion Identification Method
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DOI：
10.21273/hortsci.37.1.187
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发表时间：
2002-02
期刊：
影响因子：
1.9
通讯作者：
K. Kitahara;S. Matsumoto
中科院分区：
文献类型：
--
作者：
K. Kitahara;S. Matsumoto
从麦金托什苹果（Malus xapartica Borkh.）雌蕊中克隆了一个S等位基因cDNA。该等位基因在日本被命名为Si，在欧洲被命名为S10，是一种S-RNase，在推导的氨基酸序列水平上与S3-RNase非常相似（94%）。该等位基因可通过使用聚合酶链反应（PCR）和特异性引物扩增，然后用限制性内切酶Ehel消化来检测。在‘帝国’、‘五月柱’、‘信浓红’、‘斯宾塞’和‘维斯塔贝拉’中发现了S10等位基因。迄今为止测序的S等位基因cDNA与它们的日本和欧洲名称一起列出。
An S-allele cDNA was cloned from pistils of McIntosh' apple (Malus xdomestica Borkh.). The allele, designated S i in Japan and S 10 in Europe, is an S-RNase that is very similar (94%) to the S 3 -RNase at the deduced amino acid sequence level. This allele can be detected by amplification using the polymerase chain reaction (PCR) and specific primers, followed by digestion with restriction enzyme Ehel. The S 10 allele was discovered in 'Empire', 'Maypole', 'Shinano Red', 'Spencer', and 'Vista Bella'. The S-allele cDNAs sequenced to date are listed with their Japanese and European designations.