COMPARISON OF PROTEIN-CONTENT OF FREE AND MEMBRANE-BOUND RAT-LIVER POLYSOMES AND OF DERIVED SUBUNITS
COMPARISON OF PROTEIN-CONTENT OF FREE AND MEMBRANE-BOUND RAT-LIVER POLYSOMES AND OF DERIVED SUBUNITS
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DOI：
10.1007/bf00368299
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发表时间：
1977-01-01
影响因子：
2.8
通讯作者：
REBOUD, JP
中科院分区：
文献类型：
--
作者：
MADJAR, JJ;ARPIN, M;REBOUD, JP
采用一种高分辨率的双向凝胶电泳技术，并在第二向使用十二烷基硫酸钠，对来自纯的游离的和膜结合的大鼠肝脏多核糖体的核糖体蛋白进行了分析。在游离多核糖体中发现的三种酸性蛋白在膜结合多核糖体中始终不存在。它们的分子量约为20000、19500和18500。当游离核糖体解离为亚基时，在60S亚基图谱中仍能发现这3个蛋白斑点，但它们比在多核糖体中要弱。有人提出这3种蛋白可能参与核糖体结构与膜的附着。
Ribosomal proteins from pure free and membrane-bound rat liver polysomes were analyzed with a highly resolutive 2-dimensional gel electrophoresis technique, using sodium dodecyl sulfate in the 2nd dimension. Three acidic proteins found in free polysomes were always absent from the membrane-bound polysomes. Their MWs were about 20,000, 19,500 and 18,500. When free ribosomes were dissociated into subunits, the 3 protein spots were still found in the 60S subunit pattern, but they were weaker than in polysomes. A possible involvement of these 3 proteins in the attachment of ribosomal structures to the membranes was proposed.