Exosomes carrying mycobacterial antigens can protect mice against Mycobacterium tuberculosis infection.
Exosomes carrying mycobacterial antigens can protect mice against Mycobacterium tuberculosis infection.
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DOI：
10.1002/eji.201343727
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发表时间：
2013-12
影响因子：
5.4
通讯作者：
Schorey, Jeffery S.
中科院分区：
文献类型：
--
作者：
Cheng, Yong;Schorey, Jeffery S.
Approximately 2 billion people are infected with Mycobacterium tuberculosis, the etiological agent of tuberculosis (TB), and an estimated 1.5 million individuals die annually from TB. Presently, Mycobacterium bovis BCG remains the only licensed TB vaccine; however, previous studies suggest its protective efficacy wanes over time and fails in preventing pulmonary TB. Therefore, a safe and effective vaccine is urgently required to replace BCG or boost BCG immunizations. Our previous studies revealed that mycobacterial proteins are released via exosomes from macrophages infected with M. tuberculosis or pulsed with M. tuberculosis culture filtrate proteins (CFP). In the present study, exosomes purified from macrophages treated with M. tuberculosis CFP were found to induce antigen-specific IFN-γ and IL-2-expressing CD4+ and CD8+ T cells. In exosome-vaccinated mice there was a similar TH1 immune response but a more limited TH2 response compared to BCG vaccinated mice. Using a low-dose M. tuberculosis mouse aerosol infection model, exosomes from CFP-treated macrophages were found to both prime a protective immune response as well as boost prior BCG immunization. The protection was equal to or superior to BCG. In conclusion, our findings suggest that exosomes might serve as a novel cell-free vaccine against an M. tuberculosis infection.
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DOI：
10.1038/mt.2008.1
发表时间：
2008-04
期刊：
Molecular therapy : the journal of the American Society of Gene Therapy
影响因子：
--
作者：
Dai S;Wei D;Wu Z;Zhou X;Wei X;Huang H;Li G
通讯作者：
Li G
影响因子：
5.5
作者：
Cervantes-Villagrana AR;Hernández-Pando R;Biragyn A;Castañeda-Delgado J;Bodogai M;Martínez-Fierro M;Sada E;Trujillo V;Enciso-Moreno A;Rivas-Santiago B
通讯作者：
Rivas-Santiago B
影响因子：
3.2
作者：
Mahomed, Hassan;Fourie, P. Bernard
通讯作者：
Fourie, P. Bernard
影响因子：
3.2
作者：
Cardona, Pere-Joan
通讯作者：
Cardona, Pere-Joan
影响因子：
4.4
作者：
Dietrich, Jes;Andersen, Claire;Andersen, Peter
通讯作者：
Andersen, Peter