Increased gait variability in mice with small cerebellar cortex lesions and normal rotarod performance
Increased gait variability in mice with small cerebellar cortex lesions and normal rotarod performance
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DOI：
10.1016/j.bbr.2012.11.034
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发表时间：
2013-03-15
影响因子：
2.7
通讯作者：
D'Hooge, Rudi
中科院分区：
文献类型：
--
作者：
Stroobants, Stijn;Gantois, Ilse;D'Hooge, Rudi
小脑在神经运动表现和步态中的生理和病理生理作用是当代脑研究中的一个重要研究课题。然而，事实证明，用一些常见的行为分析方法很难测量实验室啮齿动物的细微神经运动变化和小脑功能障碍。旋转棒分析和步态分析已被广泛用作神经运动功能的指标，更具体地说，是小脑功能的指标。标准的旋转臂程序不能显示细微的运动变化，而自动步态分析在这方面可能更敏感。在本研究中，我们比较了详细的跑步机步态分析和标准加速旋转棒分析在揭示小鼠双侧小脑皮质小病变的神经运动变化方面的能力。这个小损伤模型没有明显的共济失调或异常活动的迹象。在旋转棒测试中，小脑损伤小鼠的测试水平与对照动物相同，基本步态参数没有改变。然而，小脑损伤的小鼠确实表现出前部底部宽度和后部步长变异性增加，以及不同爪子之间步长不协调的增加。我们的结论是，小脑损伤会导致步态变异性增加，就像小脑功能障碍的人类一样。跑步机步态分析比加速转杆步态分析更适合于测量这种细微的神经运动缺陷。(C)2012爱思唯尔B.V.保留所有权利。
The physiological and pathophysiological role of the cerebellum in neuromotor performance and gait is a prominent research topic in contemporary brain research. However, it has proven difficult to measure subtle neuromotor changes and cerebellar dysfunction in laboratory rodents with some of the common behavioural assays. Rotarod assays and gait analyses have been used extensively as indicators of neuromotor performance, and more specifically, cerebellar function. Standard rotarod procedures fail to reveal subtle motor alterations, whereas automated gait analysis could be more sensitive in this respect. In the present study, we compared detailed treadmill gait analysis to the standard accelerating rotarod assay in its ability to reveal neuromotor alterations in mice with small bilateral lesions in the cerebellar cortex. This small lesion model showed no readily observable signs of ataxia or abnormal activity. In the rotarod test, cerebellar-lesioned mice performed at the level of control animals, and basic gait parameters were not altered. However, cerebellar-lesioned mice did show increased front base-width and hind stride length variability, as well as increased stride length incongruity between different paws. We conclude that small cerebellar lesions lead to increased gait variability as it does in humans with cerebellar dysfunction. Treadmill gait analysis is better suited than accelerating rotarod assays to measure such subtle neuromotor defects. (C) 2012 Elsevier B.V. All rights reserved.