CHARACTERIZATION OF FOLLICULAR ENERGY-METABOLISM
CHARACTERIZATION OF FOLLICULAR ENERGY-METABOLISM
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DOI：
10.1093/oxfordjournals.humrep.a138557
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发表时间：
1994-04-01
影响因子：
6.1
通讯作者：
GOSDEN, RG
中科院分区：
文献类型：
--
作者：
BOLAND, NI;HUMPHERSON, PG;GOSDEN, RG
研究表明，小鼠卵巢卵泡具有较大的糖酵解能力，本研究旨在确定单独使用糖酵解是否能使卵泡正常发育。使用体外系统培养小鼠腔前卵泡，该系统支持在5天内发育到排卵前阶段。在氧化磷酸化抑制剂丙二酸钠存在的有氧条件下或在厌氧条件下维持培养。每24小时取出培养基样品，使用酶联免疫吸附试验（ELISA）技术分析雌二醇，使用荧光测定法分析乳酸和葡萄糖。卵泡大小、雌二醇产量和糖酵解率在对照组和丙二酸钠处理的卵泡之间无显著差异。与对照组相比，在厌氧条件下培养的卵泡的生长速度和雌二醇的产生速度明显较慢。然而，在缺氧状态下糖酵解的速率明显更高。结果表明，厌氧糖酵解可能在前心期维持有限的生长时间，但氧气的存在对确保正常发育至关重要。由此可见，单靠糖酵解作用，窦前卵泡就能发育到排卵期前，这一特点可能使它们能够为其他重要的生物合成过程保存有限的氧气供应。
It has been shown that mouse ovarian follicles have a large glycolytic capacity, and this study was undertaken to determine whether follicles can develop normally using glycolysis alone. Pre-antral mouse follicles were grown using an in-vitro system which supports development to the preovulatory stage within 5 days. Cultures were maintained in either aerobic conditions in the presence of the inhibitor of oxidative phosphorylation, sodium malonate, or under anaerobic conditions. Samples of media were removed every 24 h and analysed for oestradiol using an enzyme-linked immunosorbent assay (ELISA) technique and for lactate and glucose using a fluorometric assay. Follicle size, oestradiol production and glycolytic rate were not significantly different between control and sodium malonate-treated follicles. Follicles cultured under anaerobic conditions showed significantly slower rates of growth and oestradiol production compared with controls. However, the rate of glycolysis was significantly higher during anoxia. Results indicated that anaerobic glycolysis may sustain limited periods of growth during the pre-antral phase, but that the presence of oxygen is vital to ensure normal development. It is concluded that pre-antral follicles can undergo development to the preovulatory stage using glycolysis alone, a feature which may allow them to conserve their limited supply of oxygen for other vital biosynthetic processes.