Platelet releasate increases the proliferation and migration of bone marrow-derived cells cultured under osteogenic conditions
Platelet releasate increases the proliferation and migration of bone marrow-derived cells cultured under osteogenic conditions
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DOI：
10.1111/j.1600-0501.2005.01189.x
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发表时间：
2006-06-01
影响因子：
4.3
通讯作者：
Davies, JE
中科院分区：
文献类型：
--
作者：
Kark, LR;Karp, JM;Davies, JE
浓缩血小板及其产品目前被用作加速骨内伤口愈合的临床工具。然而，关于血小板和血小板释放产物对成骨细胞的作用知之甚少。我们在此表明，凝血酶激活的血小板的释放增加了骨髓细胞成骨培养物的迁移和增殖。使用划痕伤口试验，我们证明了血小板释放物（PR）刺激伤口闭合在无血清培养基中增加2.4 +/- 0.5倍，相对于含有凝血酶的对照。在存在血清的情况下，加入PR导致划痕闭合增加1.45 +/-0.13倍。为了将细胞迁移与细胞增殖的影响分离，将细胞单层与5、10和20 μ g/ml的丝裂霉素C（MMC）预孵育，丝裂霉素C是细胞增殖的有效抑制剂。这导致划痕闭合前沿的大幅减少，这表明PR刺激了细胞有丝分裂。然而，无论MMC预处理，PR刺激运动反应。这些结果为血小板影响骨再生的可能机制提供了证据。
Concentrated platelets and their products are currently being used as a clinical tool to accelerate endosseous wound healing. However, there is little understanding regarding the actions of platelets and platelet-released products on osteogenic cells. We show, herein, that releasate from thrombin-activated platelets increases the migration and proliferation of osteogenic cultures of bone marrow cells. Using a scratch wound assay, we demonstrated that platelet releasate (PR) stimulated up to a 2.4 +/- 0.5-fold increase in wound closure in serum-free medium, relative to a control containing thrombin. In the presence of serum, the addition of PR resulted in a 1.45 +/- 0.13-fold increase in scratch closure. To isolate cell migration from the effects of cell proliferation, cell monolayers were pre-incubated with 5, 10 and 20 mu g/ml of Mitomycin C (MMC), which is a potent inhibitor of cell proliferation. This resulted in a large decrease in the leading front of scratch closure, which indicates that PR stimulated cell mitogenesis. However, irrespective of MMC pre-treatment, PR stimulated a motogenic response. These results provide evidence of possible mechanisms by which platelets could influence bone regeneration.