Methodological Considerations for Optimizing and Validating Behavioral Assays.
Methodological Considerations for Optimizing and Validating Behavioral Assays.
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优化和验证行为测定的方法学考虑。
DOI：
10.1002/cpmo.17
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发表时间：
2016
影响因子：
--
通讯作者：
Silverman,JillL
中科院分区：
文献类型：
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作者：
SukoffRizzo,StaceyJ;Silverman,JillL
临床前动物模型是翻译研究不可缺少的工具，行为特征和表型分析对于验证假说和评估新型治疗药物治疗疾病的潜力是必不可少的。用于综合行为表型和药理学实验的方法很复杂，应由训练有素、熟练掌握的技术人员专门进行。最终目标是确定与疾病相关的、可靠的和可重复性的、可用于评估新型治疗药物治疗疾病的潜力的、与疾病相关的和可翻译的行为终点。这篇文章的目的是为建立和优化行为分析和表型电池提供一个实用的大纲，确保分析和数据是可靠的，以便它们可以在技术人员和实验室内部和之间复制，更重要的是，数据可以翻译到临床。2016John Wiley&Sons，Inc.
Preclinical animal models are indispensable tools for translational research for which behavioral characterization and phenotyping are essential to testing hypotheses and for evaluating the potential of novel therapeutic agents to treat diseases. The methods employed for comprehensive behavioral phenotyping and pharmacological experiments are complex and should be conducted exclusively by trained technicians with demonstrated proficiency. The ultimate goal is to identify disease‐relevant and translational behavioral endpoints that are robust, reliable, and reproducible, and that can be employed to evaluate potential of novel therapeutic agents to treat disease. The intent of the present article is to provide a pragmatic outline for establishing and optimizing behavioral assays and phenotyping batteries, ensuring that the assays and the data are reliable such that they can be reproduced within and across technicians and laboratories and, more importantly, that the data is translatable to the clinic. © 2016 by John Wiley & Sons, Inc.