Determination of phenolic compounds in rose hip (Rosa canina) using liquid chromatography coupled to electrospray ionisation tandem mass spectrometry and diode-array detection
Determination of phenolic compounds in rose hip (Rosa canina) using liquid chromatography coupled to electrospray ionisation tandem mass spectrometry and diode-array detection
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DOI：
10.1002/rcm.622
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发表时间：
2002-01-01
影响因子：
2
通讯作者：
Hvattum, E
中科院分区：
文献类型：
--
作者：
Hvattum, E
采用液相色谱-电喷雾离子化（ESI）串联质谱（MS/MS）和二极管阵列检测器（DAD）联用技术测定犬蔷薇果提取物中的酚类化合物。色谱分离的酚类的ESI质谱给出了化合物的分子量，通过突出的[M-H]（-）离子的化合物和花青素苷的M+离子。[M-H]（-）（或M+）前体离子的碰撞诱导解离（CID）产生决定糖苷配基分子量的产物离子。源内裂解，然后对所得的去质子化糖苷配基（[A-H]（-））进行CID，为非共轭酚类的鉴定提供了产物离子。鉴别基于与商业标准品的产物离子光谱的比较。UV-二极管阵列光谱用于鉴别确认。这种组合的方法允许在玫瑰果提取物中鉴定花色素苷，即矢车菊素-3-0-葡萄糖苷、槲皮素的几种糖苷以及花旗松素和圣草酚的糖苷。鉴定了根皮苷，还发现了几种没食子酸甲酯的结合物，其中一种初步鉴定为没食子酸甲酯-芸香糖苷。儿茶素和槲皮素被发现作为提取物中的糖苷配基。版权所有（C）2002约翰威利父子有限公司
Liquid chromatography coupled with negative and positive electrospray ionisation (ESI) tandem mass spectrometry (MS/MS) and diode-array detection (DAD) was used for determination of phenols in rose hip (Rosa canina) extract. ESI mass spectra of the chromatographically separated phenols gave the molecular weight of the compounds through prominent [M-H](-) ions for most of the compounds and M+ ions for the anthocyanins. Collision induced dissociation (CID) of the [M-H](-) (or M+) precursor ions yielded product ions which determined the molecular weight of the aglycones. In-source fragmentation followed by CID of the resulting deprotonated aglycone ([A-H](-)) provided product ions for the identification of the unconjugated phenols. The identification was based on comparison with product ion spectra of commercial standards. UV-diode-array spectra were used for identity confirmation. This combined approach allowed the identification in rose hip extract of an anthocyanin, i.e. cyanidin-3-O-glucoside, several glycosides of quercetin and glycosides of taxifolin and eriodictyol. Phloridzin was identified, and several conjugates of methyl gallate were also found, one of which was tentatively identified as methyl gallate-rutinoside. Catechin and quercetin were found as the aglycones in the extract. Copyright (C) 2002 John Wiley Sons, Ltd.