6-Chloro-N,N-diethyl-1,3,5-triazine-2,4-diamine (CAT) Sensor Based on Biomimetic Recognition Utilizing a Molecularly Imprinted Artificial Receptor
6-Chloro-N,N-diethyl-1,3,5-triazine-2,4-diamine (CAT) Sensor Based on Biomimetic Recognition Utilizing a Molecularly Imprinted Artificial Receptor
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DOI：
10.2116/analsci.23.49
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发表时间：
2007-01
影响因子：
1.6
通讯作者：
Yusuke Fuchiwaki;A. Shimizu;I. Kubo
中科院分区：
文献类型：
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作者：
Yusuke Fuchiwaki;A. Shimizu;I. Kubo
我们的目标是开发6-氯- N，N -二乙基-1，3，5-三嗪-2，以甲基丙烯酸（MAA）为功能单体，乙二醇二甲基丙烯酸酯（EDMA）为交联剂，合成了一种4-二胺（CAT）的分子印迹聚合物，并将其用于CAT的电化学检测。在二甲基甲酰胺（DMF）中，将连接子与模板分子（CAT）连接。以CAT：MAA：EDMA = 1：7.5：20的比例制备的聚合物对CAT具有最高的选择性结合能力，并将所得聚合物鉴定为CAT印迹聚合物（CAT-MIP）。通过Scatchard分析证明了CAT-MIP的特异性印迹位点的作用。在CAT的水溶液中，CAT-MIP在0.05 M磷酸盐缓冲液（PB），pH 5.0中显示CAT的最大结合。CAT与CAT-MIP的结合量比莠去津高24%，比氯丙嗪高72%。CAT传感系统由CAT-MIP颗粒柱和伏安分析仪组成。在30 μM范围内，CAT的还原电流依赖于CAT的浓度。
We aimed to develop a 6-chloro- N , N -diethyl-1,3,5-triazine-2,4-diamine (CAT)-sensing system based on a biomimetic receptor of a molecularly imprinted polymer for CAT and electrochemical determination of CAT. A molecularly imprinted polymer for CAT was prepared by the polymerization of methacrylic acid (MAA) as a functional monomer and ethylene glycol dimethacrylate (EDMA) as a cross-linker with a template molecule (CAT) in dimethyl formamide (DMF). The polymer prepared with the ratio of these monomers (CAT:MAA:EDMA = 1:7.5:20) showed the most selective rebinding to CAT, and the obtained polymer was recognized as a CAT-imprinted polymer (CAT-MIP). The effect of the specific imprinting sites of CAT-MIP was demonstrated by Scatchard analysis. In an aqueous solution of CAT, CAT-MIP showed the maximum binding of CAT in a 0.05 M phosphate buffer (PB), pH 5.0. The binding amount of CAT to CAT-MIP was 24% more than atrazine and 72% more than propazine. The CAT-sensing system was composed of a column of CAT-MIP particles and a voltammetry analyzer. The reductive current of CAT depended on the concentration of CAT up to 30 μM with the system.