Symbiotic germination of three semi-aquatic rein orchids (Habenaria repens, H-quinquiseta, H-macroceratitis) from Florida
Symbiotic germination of three semi-aquatic rein orchids (Habenaria repens, H-quinquiseta, H-macroceratitis) from Florida
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DOI：
10.1016/s0304-3770(01)00214-5
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发表时间：
2002-02-01
期刊：
影响因子：
1.8
通讯作者：
Zettler, LW
中科院分区：
文献类型：
--
作者：
Stewart, SL;Zettler, LW
人口稠密地区（例如佛罗里达）湿地的破坏引发了人们对栖息地恢复的兴趣。我们描述了一种共生技术，使来自佛罗里达的三种半水生雷鸟兰物种（Habenaria repens，H. quinquiseta，五毛草H. macroceratitis）和培养H.匍匐茎幼苗离体培养。所有三种玉凤花属的种子。在接种的21天内发芽。结果表明，H.利用两个真菌分离物（Epulorhiza spp.）恢复前佛罗里达兰花Spiranthes brevilabris和Epidendrum conopseum。感染S.在温室中转移到泥炭中的短唇霉真菌具有最高的存活率（88.9%）（体外>159天）。一个H种子播种后18个月开始开花。这项研究概述的方法有可能被湿地恢复项目采用，寻求包括兰花（H。repens）和菌根真菌作为生物制剂。(C)2002 Elsevier Science B.V.保留所有权利。
The destruction of wetlands in populated areas (e.g. Florida) has prompted interest in habitat restoration, We describe a symbiotic technique to germinate seeds of three semi-aquatic rein orchid species from Florida (Habenaria repens, H. quinquiseta, H. macroceratitis) and to cultivate H. repens seedlings on soil ex vitro. Seeds of all three Habenaria spp. germinated within 21 days of inoculation. Leaf-bearing seedlings of H. repens were obtained using two fungal isolates (Epulorhiza spp.) recovered front Florida orchids Spiranthes brevilabris and Epidendrum conopseum. Seedlings infected with the S. brevilabris fungus that were transferred to peat in a greenhouse had the highest (88.9%) survival (>159 days ex vitro). One H. repens seedling initiated anthesis 18 months after seed sowing. The methods outlined by this study have the potential to be adopted by wetland restoration projects seeking to include an orchid (H. repens) and mycorrhizal fungi as biotic agents. (C) 2002 Elsevier Science B.V. All rights reserved.