THE C-ELEGANS GENE LIN-44, WHICH CONTROLS THE POLARITY OF CERTAIN ASYMMETRIC CELL DIVISIONS, ENCODES A WNT PROTEIN AND ACTS CELL NONAUTONOMOUSLY
THE C-ELEGANS GENE LIN-44, WHICH CONTROLS THE POLARITY OF CERTAIN ASYMMETRIC CELL DIVISIONS, ENCODES A WNT PROTEIN AND ACTS CELL NONAUTONOMOUSLY
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DOI：
10.1016/0092-8674(95)90238-4
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发表时间：
1995-10-06
期刊：
影响因子：
64.5
通讯作者：
HERMAN, RK
中科院分区：
文献类型：
--
作者：
HERMAN, MA;VASSILIEVA, LL;HERMAN, RK
线虫基因LIN-44的突变会导致某些不对称细胞分裂的极性逆转。我们已经发现，LIN-44是WNT家族的成员，该家族编码与细胞间信号转导有关的分泌性糖蛋白。使用LIN-44转录本的原位杂交实验和使用旨在模拟LIN-44表达模式的报告构建体的实验都表明，LIN-44表达在尾端的皮下细胞中，位于受到LIN-44突变影响的极性细胞的后面。我们的马赛克分析表明，LIN-44的作用是非自主的。我们认为，LIN-44蛋白由尾部皮下细胞分泌，并影响尾部更靠前的不对称细胞分裂的极性。
Mutations in the C. elegans gene lin-44 lead to reversals in the polarity of certain asymmetric cell divisions. We have discovered that lin-44 is a member of the Wnt family of genes, which encode secretory glycoproteins implicated in intercellular signaling. Both in situ hybridization experiments using lin-44 transcripts and experiments using reporter constructs designed to mimic patterns of lin-44 expression indicate that lin-44 is expressed in hypodermal cells at the tip of the tail and posterior to the cells with polarities affected by lin-44 mutations. Our mosaic analysis indicates that lin-44 acts cell nonautonomously. We propose that LIN-44 protein is secreted by tail hypodermal cells and affects the polarity of asymmetric cell divisions that occur more anteriorly in the tail.