Purgative/laxative actions of Globularia alypum aqueous extract on gastrointestinal-physiological function and against loperamide-induced constipation coupled to oxidative stress and inflammation in rats
Purgative/laxative actions of Globularia alypum aqueous extract on gastrointestinal-physiological function and against loperamide-induced constipation coupled to oxidative stress and inflammation in rats
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DOI：
10.1111/nmo.13858
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发表时间：
2020-04-26
影响因子：
3.5
通讯作者：
Sebai, Hichem
中科院分区：
文献类型：
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作者：
Hajji, Najla;Wannes, Dalanda;Sebai, Hichem
背景慢性便秘是一种影响患者生活质量的胃肠功能障碍。因此，许多研究都致力于寻找草药泻药。在这项研究中，我们研究了球芽孢杆菌叶水提取物 (GAAE) 对洛哌丁胺 (LOP) 引起的便秘的作用。 方法 给动物服用 LOP（3 毫克/公斤，体重，腹腔注射）和 GAAE（100、200 和 400 毫克/公斤，体重，口服）或育亨宾（2 毫克/公斤，体重，口服）。 i.p.), 同时，持续1周。测定胃排空试验和肠道运输。检查结肠组织学，并使用生化比色法评估氧化状态。 主要结果 GAAE 显着改善胃排空（64% 至 76.5%）和肠道转运（66.65% 至 84.73%）。 LOP 对排便参数产生负面影响并产生压力情况。相比之下，GAAE 给药改善了这些参数并重新建立了氧化平衡。结论 GAAE 显示出适度的抗氧化应激作用并降低 LOP 效应，因此可以被认为是治疗便秘的药物。
Background Chronic constipation is a gastrointestinal functional disorder which affects patient quality of life. Therefore, many studies were oriented to search herbal laxative agents. In this study, we investigated the effect of Globularia alypum L. leaves aqueous extract (GAAE) against loperamide (LOP)-produced constipation.Methods Animals were given LOP (3 mg/kg, b.w., i.p.) and GAAE (100, 200, and 400 mg/kg, b.w., p.o.) or yohimbine (2 mg/kg, b.w., i.p.), simultaneously, for 1 week. Gastric-emptying test and intestinal transit were determined. Colon histology was examined, and oxidative status was evaluated using biochemical-colorimetric methods.Key Results GAAE ameliorates significantly gastric emptying (64% to 76.5%) and intestinal transit (66.65% to 84.73%). LOP negatively influenced defecation parameters and generated a stress situation. GAAE administration in contrast ameliorated those parameters and re-established oxidative balance.Conclusion GAAE showed a modest action against oxidative stress and decreased LOP effect and thereby can be considered a pharmacological agent in constipation.