Phylogenetic Analysis of 16S Ribosomal RNA Gene Sequences from Human Fecal Microbiota and Improved Utility of Terminal Restriction Fragment Length Polymorphism Profiling
Phylogenetic Analysis of 16S Ribosomal RNA Gene Sequences from Human Fecal Microbiota and Improved Utility of Terminal Restriction Fragment Length Polymorphism Profiling
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人类粪便微生物群 16S 核糖体 RNA 基因序列的系统发育分析以及末端限制性片段长度多态性分析的改进实用性
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
K. Shimomura
中科院分区：
文献类型：
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作者：
K. Nagashima;J. Mochizuki;Takayoshi Hisada;Shuji Suzuki;K. Shimomura
我们已经开发了一种末端限制性片段长度多态性（T-RFLP）的方法来确定肠道微生物群落的结构和动态。本文介绍了改进的T-RFLP方法，并结合对6个个体粪便16 SrDNA（核糖体RNA基因）克隆文库的分析。共测定了418个不同的16 S rDNA部分序列，并进行了系统发育分析和同源性检查。我们发现这些序列大致可分为6个系统发育群，包含7个亚群，与71个已知物种相关，相似性超过90%。除少数情况外，我们发现，在二氧化硅中，属于相同细菌组或亚组的序列的BstI-消化产生了相似长度的末端限制性片段。我们的结论是，人类肠道菌群主要由大约10个系统发育细菌群的成员组成，这些细菌群是有效区分我们的T-RFLP系统。
We have developed a terminal restriction fragment length polymorphism (T-RFLP) method for determining the structure and dynamics of the microbial gut community. In this paper, the improved T-RFLP method in combination with an analysis of the fecal 16S rDNA (ribosomal RNA gene) clone libraries from six individuals is described. A total of 418 different partial sequences of 16S rDNA were determined and subjected to a phylogenetic analysis and homology examination. We found that the sequences were roughly divided into six phylogenetic groups containing seven subgroups and were related to 71 known species with over 90% similarity. With the exception of a few cases, we found that in silica BstI-digestioa of the sequences belonging to the same bacterial group or subgroup generated terminal restriction fragments of similar lengths. We concluded that human intestinal microbiota predominantly consists of the members of approximately ten phylogenetic bacterial groups and that these bacterial groups are effectively distinguished by our T-RFLP system.