Correlating 3D morphology with molecular pathology: fibrotic remodelling in human lung biopsies
Correlating 3D morphology with molecular pathology: fibrotic remodelling in human lung biopsies
复制标题
将 3D 形态学与分子病理学相关联：人肺活检中的纤维化重塑
DOI：
10.1136/thoraxjnl-2015-207131
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发表时间：
2015
期刊：
影响因子：
10
通讯作者：
Matthias
中科院分区：
文献类型：
--
作者：
Manuela Kellner;Judith Wehling;Gregor Warnecke;Marko Heidrich;Nicole Izykowski;Jens Vogel-Claussen;Raoul-Amadeus Lorbeer;Georgios Antonopoulos;Sabina Janciauskiene;Roman Grothausmann;Lars Knudsen;Tammo Ripken;Heiko Meyer;Hans Kreipe;Matthias
评估肺实质结构的改变对于理解纤维化间质性肺疾病是至关重要的。在这里，我们提出了一种新的方法来可视化人类肺部的纤维化重塑，并将形态学三维（3D）数据与同一样本中的基因和蛋白质表达相关联。我们的方法的关键是一种新型的包埋树脂，可以将样品清除到完全的光学透明度，同时允许3D激光断层扫描和组织学，免疫组织化学和RNA分离切片的制备。将3D激光断层扫描与分子诊断技术相结合，可以为肺部疾病提供新的见解。这种方法有很大的潜力成为肺研究的一个重要工具。
Assessing alterations of the parenchymal architecture is essential in understanding fibrosing interstitial lung diseases. Here, we present a novel method to visualise fibrotic remodelling in human lungs and correlate morphological three-dimensional (3D) data with gene and protein expression in the very same sample. The key to our approach is a novel embedding resin that clears samples to full optical transparency and simultaneously allows 3D laser tomography and preparation of sections for histology, immunohistochemistry and RNA isolation. Correlating 3D laser tomography with molecular diagnostic techniques enables new insights into lung diseases. This approach has great potential to become an essential tool in pulmonary research.