Sensitivity and specificity of a lateral flow immunoassay (LFI) in serum samples for diagnosis of melioidosis.
Sensitivity and specificity of a lateral flow immunoassay (LFI) in serum samples for diagnosis of melioidosis.
复制标题
DOI：
10.1093/trstmh/try099
复制
发表时间：
2018-12-01
影响因子：
2.2
通讯作者：
Limmathurotsakul D
中科院分区：
文献类型：
--
作者：
Wongsuvan G;Hantrakun V;Teparrukkul P;Imwong M;West TE;Wuthiekanun V;Day NPJ;AuCoin D;Limmathurotsakul D
培养是诊断类鼻疽（一种由类鼻疽伯克霍尔德杆菌引起的感染）的金标准。在这里，我们评估了侧流免疫分析（LFI）来检测血清样本中的类鼻疽杆菌荚膜多糖（CPS）。选择从任何临床标本中培养出类鼻疽伯克氏菌呈阳性的患者作为病例。选择血培养金黄色葡萄球菌、大肠杆菌或肺炎克雷伯菌阳性的患者以及疟疾或登革热聚合酶链反应检测阳性的患者作为对照。血清样本中 LFI 的敏感性为 31.3%（60/192 病例患者 [95% 置信区间 {CI} 24.8 至 38.3]），特异性为 98.8%（559/566 对照患者 [95% CI 97.4 至 99.5]）。尽管 LFI 在血清中的敏感性可能有限，但它可以在资源有限的环境中快速诊断类鼻疽。
Culture is the gold standard for the diagnosis of melioidosis, an infection caused by Burkholderia pseudomallei. Here we evaluate a lateral flow immunoassay (LFI) to detect B. pseudomallei capsular polysaccharide (CPS) in serum samples. Patients with culture from any clinical specimen positive for B. pseudomallei were selected as cases. Patients who were blood culture positive for Staphylococcus aureus, Escherichia coli or Klebsiella pneumoniae as well as those who were malaria or dengue polymerase chain reaction assay positive were selected as controls. The sensitivity of the LFI was 31.3% (60/192 case patients [95% confidence interval {CI} 24.8 to 38.3]) and the specificity was 98.8% (559/566 control patients [95% CI 97.4 to 99.5]) in serum samples. Although LFI may have limited sensitivity in serum, it can rapidly diagnose melioidosis in resource-limited settings.
登录
查看更多内容
影响因子：
3.8
作者：
Houghton, Raymond L.;Reed, Dana E.;AuCoin, David P.
通讯作者：
AuCoin, David P.
影响因子：
2.9
作者：
Hinjoy S;Hantrakun V;Kongyu S;Kaewrakmuk J;Wangrangsimakul T;Jitsuronk S;Saengchun W;Bhengsri S;Akarachotpong T;Thamthitiwat S;Sangwichian O;Anunnatsiri S;Sermswan RW;Lertmemongkolchai G;Tharinjaroen CS;Preechasuth K;Udpaun R;Chuensombut P;Waranyasirikul N;Anudit C;Narenpitak S;Jutrakul Y;Teparrukkul P;Teerawattanasook N;Thanvisej K;Suphan A;Sukbut P;Ploddi K;Sirichotirat P;Chiewchanyon B;Rukseree K;Hongsuwan M;Wongsuwan G;Sunthornsut P;Wuthiekanun V;Sachaphimukh S;Wannapinij P;Chierakul W;Chewapreecha C;Thaipadungpanit J;Chantratita N;Korbsrisate S;Taunyok A;Dunachie S;Palittapongarnpim P;Sirisinha S;Kitphati R;Iamsirithaworn S;Chaowagul W;Chetchotisak P;Whistler T;Wongratanacheewin S;Limmathurotsakul D
通讯作者：
Limmathurotsakul D
影响因子：
28.3
作者：
Limmathurotsakul, Direk;Golding, Nick;Hay, Simon I.
通讯作者：
Hay, Simon I.
DOI：
10.4269/ajtmh.2007.77.972
发表时间：
2007-11-01
影响因子：
3.3
作者：
Paris, Daniel H.;Imwong, Mallika;Dondorp, Arjen M.
通讯作者：
Dondorp, Arjen M.
影响因子：
3.7
作者：
Teparrukkul P;Hantrakun V;Day NPJ;West TE;Limmathurotsakul D
通讯作者：
Limmathurotsakul D