Field testing of a putative larviposition pheromone for the tsetse fly Glossina m. morsitans Westwood
Field testing of a putative larviposition pheromone for the tsetse fly Glossina m. morsitans Westwood
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DOI：
10.4001/003.029.0635
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发表时间：
2021-09-01
影响因子：
0.8
通讯作者：
Saini, R. K.
中科院分区：
文献类型：
--
作者：
Hargrove, J. W.;Van Sickle, J.;Saini, R. K.
在实验室条件下，烷烃正十五烷吸引怀孕的雌性采采蝇(Glossina Morsitans Morsitans Westwood)到潜在的幼虫产卵地点。我们描述了这种化学物质的田间试验，这是第一次对任何假定的采采蝇幼虫信息素进行这样的试验。我们在津巴布韦赞比西河谷Rekomitjie研究站附近的11个地点重复了随机区组实验。每个地点都由四个用200升钢桶建造的人工疣猪洞组成。我们在每个洞穴里放了六个托盘，里面装满了沙子，上面覆盖着树叶垃圾。在实验期间，栖息在这一地区的两种采采蝇都进入了这些人工洞穴，并在托盘中放置了1282只幼虫。在13个实验中，正十五烷在三个不同的浓度下与对照组进行了测试。我们无法找到任何确凿的证据表明，正十五烷的存在导致了变态杆菌或苍白球虫幼虫存活率的增加。这一发现与使用的正十五烷浓度无关，也不受添加水、丙酮或疣猪尿的存在或不存在的影响。信息素的任何微小影响都可能被洞穴幼虫位置的重要物理特征所掩盖。在幼虫取食地点添加枯枝落叶后，幼虫率的增加幅度远大于使用假定的幼虫信息素。
D Under laboratory conditions, the alkane hydrocarbon n-pentadecane attracts gravid female tsetse (Glossina morsitans morsitans Westwood) to potential larviposition sites. We describe field-testing of this chemical, which is the first such test for any putative tsetse larviposition pheromone. We replicated randomised block experiments at 11 sites near Rekomitjie Research Station, Zambezi Valley, Zimbabwe. Each site comprised a cluster of four artificial warthog burrows constructed from 200-litre steel drums. We placed six trays, filled with sand and covered with leaf litter, inside each burrow. Both species of tsetse inhabiting this region entered these artificial burrows and deposited 1282 pupae in the trays during the experiments. In 13 experiments, n-pentadecane was tested at three different concentrations against a control. We were unable to find any conclusive evidence that the presence of n-pentadecane resulted in an increased larviposition rate for either G. m. morsitans or G. pallidipes. This finding was independent of the concentration of n-pentadecane used, and was not influenced by the presence or absence of added water, acetone or warthog urine. Any small effect of the pheromone is likely masked by the important physical characteristics of burrow larviposition sites. Order of magnitude increases in larviposition rates when leaf litter was added to larviposition sites were far greater than those due to the use of putative larviposition pheromones.