Biocompatible evaluation of barium titanate foamed ceramic structures for orthopedic applications
Biocompatible evaluation of barium titanate foamed ceramic structures for orthopedic applications
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DOI：
10.1002/jbm.a.34879
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发表时间：
2014-07-01
影响因子：
4.9
通讯作者：
Allen, Josephine B.
中科院分区：
文献类型：
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作者：
Ball, Jordan P.;Mound, Brittnee A.;Allen, Josephine B.
钛酸钡(BT)的电活性使其成为再生医学中感兴趣的材料。为了加深对该材料在骨科应用的了解，采用直接发泡技术制备了多孔BT，并对其体外生物相容性进行了研究。对合成的泡沫进行了表征，总孔隙率在50%到70%之间，平均孔径超过30微米。将Bt与小鼠成骨细胞(7F2)细胞共培养，用乳酸脱氢酶(LDH)法测定泡沫的毒性程度。72小时后，BT泡沫塑料的细胞毒性为6.4+/-0.8%，与多孔45S5生物玻璃(R)的8.4+/-1.5%相当。用人单核细胞白血病细胞株(THP-1)分泌的肿瘤坏死因子-α(TNF-α)作为体外炎症反应的函数，测定了多孔BT诱导的体外炎症反应。结果表明，BT泡沫没有引起明显的炎症反应，每毫升培养基能产生9.4+/-1.3pg的肿瘤坏死因子-α，而未经处理的细胞则为20.2+/-2.3pg/ml。这些结果表明，多孔BT没有短期的细胞毒性，具有骨科组织工程应用的潜力。(C)2013年威利期刊公司。
The potential of barium titanate (BT) to be electrically active makes it a material of interest in regenerative medicine. To enhance the understanding of this material for orthopedic applications, the in vitro biocompatibility of porous BT fabricated using a direct foaming technique was investigated. Characterization of the resultant foams yielded an overall porosity between 50 and 70% with average pore size in excess of 30 mu m in diameter. A mouse osteoblast (7F2) cell line was cultured with the BT to determine the extent of the foams' toxicity using a LDH assay. After 72 h, BT foams showed a comparable cytotoxicity of 6.4 +/- 0.8% to the 8.4 +/- 1.5% of porous 45S5 Bioglass (R). The in vitro inflammatory response elicited from porous BT was measured as a function of tumor necrosis factor alpha (TNF-alpha) secreted from a human monocytic leukemia cell line (THP-1). Results indicate that the BT foams do not cause a significant inflammatory response, eliciting a 9.4 +/- 1.3 pg of TNF-alpha per mL of media compared with 20.2 +/- 2.3 pg/mL from untreated cells. These results indicate that porous BT does not exhibit short term cytotoxicity and has potential for orthopedic tissue engineering applications. (C) 2013 Wiley Periodicals, Inc.