Hemoglobin is expressed in alveolar epithelial type II cells
Hemoglobin is expressed in alveolar epithelial type II cells
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DOI：
10.1016/j.bbrc.2005.06.042
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发表时间：
2005-08-12
影响因子：
3.1
通讯作者：
Liu, L
中科院分区：
文献类型：
--
作者：
Bhaskaran, M;Chen, HF;Liu, L
血红蛋白是红细胞中主要的携氧血红素蛋白。为了利用DNA芯片技术研究肺泡上皮细胞I型和II型的差异基因表达，我们发现Hbα和β链的mRNAs在II型细胞中表达，而在I型细胞中不表达。用RT-PCR对芯片数据进行验证。当II型细胞反式分化为I型样细胞时，这两条链的mRNA表达均下降。免疫细胞/组织化学显示，血红蛋白特异性地定位于肺细胞混合物的II型细胞和大鼠肺组织。肺泡上皮细胞内源性合成血红蛋白提示，除肺内氧气转运外，血红蛋白可能还具有未知的功能。(C)2005 Elsevier Inc.保留所有权利。
Hemoglobin is the main oxygen carrying heme protein in erythrocytes. In an effort to study the differential gene expression of alveolar epithelial type I and type II cells using DNA microarray technique, we found that the mRNAs of hemoglobin alpha- and beta-chains were expressed in type II cells, but not in type I cells. The microarray data were confirmed by RT-PCR. The mRNA expression of both chains decreased when type II cells trans-differentiated into type I-like cells. Immunocyto/histochemistry revealed that hemoglobin protein was specifically localized in type II cells of a lung cell mixture and rat lung tissue. The endogenous synthesis of hemoglobin in alveolar epithelial cells suggests that hemoglobin may have unidentified functions other than oxygen transport in the lung. (c) 2005 Elsevier Inc. All rights reserved.