IncRNA-RMRP promotes proliferation, migration and invasion of bladder cancer via miR-206
IncRNA-RMRP promotes proliferation, migration and invasion of bladder cancer via miR-206
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DOI：
10.26355/eurrev_201902_16988
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发表时间：
2019-02-01
影响因子：
3.3
通讯作者：
Xi, J. -N.
中科院分区：
文献类型：
--
作者：
Cao, H. -L.;Liu, Z. -J.;Xi, J. -N.
目的：膀胱癌在世界范围内发病率很高，但其发生发展的详细机制尚不清楚。近年来，长链非编码RNA（IncRNA）在许多疾病中发挥着重要作用。本研究主要探讨线粒体RNA加工核糖核酸内切酶（IncRNA-RMRP）的RNA组分在膀胱癌中的作用。材料与方法：采用qRT-PCR方法检测IncRNA-RMRP在膀胱癌患者和肿瘤细胞中的表达。并分析其临床意义。MTT法检测细胞增殖活性，transwell法检测细胞迁移和侵袭能力。在抑制IncRNA RMRP后。Western blot检测转染后膀胱癌细胞中IncRNA RMRP蛋白的相对表达水平。结果：与癌旁组织相比，IncRNA RMRP在膀胱癌组织中高表达。RMRP的表达与肿瘤的大小、淋巴结转移及生存期密切相关。结论：IncRNA RMRP与膀胱癌的发生发展密切相关，可能成为治疗膀胱癌的潜在靶点。
OBJECTIVE: The incidence of bladder cancer (BC) is common in the world, but its detail mechanisms for occurrence and development remain unclear. Recently, long non-coding RNAs (IncRNAs) have been observed to play an important role in many different diseases. In this research, we mainly explored the role of the RNA component of mitochondrial RNA processing endoribonuclease (IncRNA-RMRP) in bladder cancer.MATERIALS AND METHODS: We used qRT-PCR to detect the expression of IncRNA-RMRP in bladder cancer patients and tumor cells. and the clinical significance was also analyzed. The methyl thiazolyl tetrazolium (MTT) assay was used to detect the cell proliferation, and we used transwell to detect the migration and invasion. after the IncRNA RMRP was inhibited. Western-blot was used to measure the relative protein expression level in bladder cancer cells after transfection with siRNA-NC or siRNA-RMRP.RESULTS: We found that the IncRNA RMRP was highly expressed in bladder cancer tissue, compared with adjacent tissue. We also found that the expression of RMRP was closely related with the size, lymph node metastasis and survival time of patients. What's more, RMRP could promote the proliferation, migration and invasion of BC cell lines via regulating miR-206 as a sponge.CONCLUSIONS: According to the results, we found that IncRNA RMRP was closely related to the progression of bladder cancer, which could be a potential target for treating BC patients.