Anti-fatigue activity of a novel polysaccharide conjugates from Ziyang green tea
Anti-fatigue activity of a novel polysaccharide conjugates from Ziyang green tea
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DOI：
10.1016/j.ijbiomac.2015.06.055
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发表时间：
2015-09-01
影响因子：
8.2
通讯作者：
Tang, Liang
中科院分区：
文献类型：
--
作者：
Chi, Aiping;Li, Hong;Tang, Liang
研究紫阳绿色茶多糖的抗疲劳活性。从资阳绿色茶中提取多糖，并对其理化性质进行分析。同时，建立小鼠4周负重游泳实验，并在运动过程中口服多糖。测定力竭时间、血尿素氮（BUN）、血乳酸（Bla）、血清乳酸脱氢酶（LDH）活性、骨骼肌超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性、丙二醛（MDA）和糖原含量。结果表明，紫阳绿色茶多糖是一种硒-多糖-蛋白质结合物（Se-TP），硒-TP能显著延长力竭时间，提高骨骼肌糖原水平和GSH-Px活性，降低血清BUN、Bla水平和LDH活性，降低骨骼肌MDA水平。结论：硒茶多酚能显著改善运动性疲劳，降低力竭运动引起的氧化应激。(C)2015 Elsevier B. V.版权所有。
The aim of this study was to investigate the anti-fatigue activity of polysaccharides from Ziyang green tea. Polysaccharides were isolated from Ziyang green tea and its physicochemical properties were analyzed. Meanwhile, a 4-week weight-loaded swimming test of mice was established and polysaccharides were orally administrated during exercise. The biochemical parameters related to fatigue were determined, such as exhaustive time, blood urea nitrogen (BUN), blood lactate acid (Bla) levels and lactic dehydrogenase (LDH) activity in serum, Superoxide dismutase (SOD), Glutathione peroxidase (GSH-Px) activities, Malondialdehyde (MDA) and glycogen levels in skeletal muscle. The results demonstrated that polysaccharide from Ziyang green tea was a selenium-polysaccharide-protein conjugate (Se-TP), and Se-TP administration significantly prolonged exhaustive time and increased glycogen level and GSH-Px activity in muscle, in addition, markedly decreased BUN, Bla levels and LDH activity in serum and MDA level in muscle. In conclusion, Se-TP treatment can significantly improve exercise-induced fatigue and decrease the oxidative stress induced by the exhaustive exercise. (C) 2015 Elsevier B.V. All rights reserved.