Qualitative Characterization of the Rat Liver Mitochondrial Lipidome using LC-MS Profiling and High Energy Collisional Dissociation (HCD) All Ion Fragmentation.
Qualitative Characterization of the Rat Liver Mitochondrial Lipidome using LC-MS Profiling and High Energy Collisional Dissociation (HCD) All Ion Fragmentation.
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DOI：
10.1007/s11306-012-0400-1
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发表时间：
2013-03
期刊：
影响因子：
3.6
通讯作者：
Kristal, Bruce S.
中科院分区：
文献类型：
--
作者：
Bird, Susan S.;Marur, Vasant R.;Stavrovskaya, Irina G.;Kristal, Bruce S.
脂质在线粒体正常功能中扮演着重要的多重角色，包括参与膜结构和流动性、细胞能量储存和信号传导。脂质也是活性物质的主要目标，它们的过氧化副产物本身介导进一步的损害。来自多个类别和类别的数千种脂质物质参与这些过程，这表明脂质定量和结构分析可以帮助更好地了解线粒体的生理状态。由于有助于和反映线粒体功能的脂质的多样性，理想情况下，分析方法应涵盖广泛的脂质类别，并产生定量和结构信息。我们开发了一种高分辨率LC-MS方法，能够监测生物标本中发现的主要脂质类（即，生物流体、细胞和组织）以有效、灵敏和稳健的方式进行相对定量，同时还通过所有离子HCD片段化和色谱比对表征单个脂质侧链。这种方法被用来配置文件的肝脏线粒体脂质从192只大鼠进行膳食常量营养素的研究，其中线粒体功能的变化与每种饮食的主要脂肪和血糖指数成分的变化。使用非靶向LC-MS分析，在阳性和阴性模式下，在线粒体中鉴定了总共381种独特脂质，跨越5种主要LIPID MAPS定义的类别，包括脂肪酰、甘油磷脂、甘油酯、鞘脂和异戊烯醇。本报告的目的是显示这种非靶向LC-MS分析方法在分析、鉴别和表征线粒体脂质组的能力方面的广度，并将讨论其细节。
Lipids play multiple roles essential for proper mitochondrial function, from their involvement in membrane structure and fluidity, cellular energy storage, and signaling. Lipids are also major targets for reactive species, and their peroxidation byproducts themselves mediate further damage. Thousands of lipid species, from multiple classes and categories, are involved in these processes, suggesting lipid quantitative and structural analysis can help provide a better understanding of mitochondrial physiological status. Due to the diversity of lipids that contribute to and reflect mitochondrial function, analytical methods should ideally cover a wide range of lipid classes, and yield both quantitative and structural information. We developed a high resolution LC-MS method that is able to monitor the major lipid classes found in biospecimens (ie. biofluids, cells and tissues) with relative quantitation in an efficient, sensitive, and robust manner while also characterizing individual lipid side-chains, by all ion HCD fragmentation and chromatographic alignment. This method was used to profile the liver mitochondrial lipids from 192 rats undergoing a dietary macronutrient study in which changes in mitochondria function are related to changes in the major fat and glycemic index component of each diet. A total of 381 unique lipids, spanning 5 of the major LIPID MAPS defined categories, including fatty acyls, glycerophospholipids, glycerolipids, sphingolipids and prenols, were identified in mitochondria using the non-targeted LC-MS analysis in both positive and negative mode. The intention of this report is to show the breadth of this non-targeted LC-MS profiling method with regards to its ability to profile, identify and characterize the mitochondrial lipidome and the details of this will be discussed.
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影响因子：
7.4
作者：
Bird, Susan S.;Marur, Vasant R.;Sniatynski, Matthew J.;Greenberg, Heather K.;Kristal, Bruce S.
通讯作者：
Kristal, Bruce S.
影响因子：
3.6
作者：
BARZANTI, V;BATTINO, M;LENAZ, G
通讯作者：
LENAZ, G
影响因子：
2.9
作者：
Han, XL;Yang, JY;Gross, RW
通讯作者：
Gross, RW
影响因子：
7.4
作者：
CHEN, JJ;YU, BP
通讯作者：
YU, BP
影响因子：
4.8
作者：
Gudz, TI;Tserng, KY;Hoppel, CL
通讯作者：
Hoppel, CL