Comparison of the gene expression profiles of resistant and non-resistant Japanese black pine inoculated with pine wood nematode using a modified LongSAGE technique
Comparison of the gene expression profiles of resistant and non-resistant Japanese black pine inoculated with pine wood nematode using a modified LongSAGE technique
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DOI：
10.1111/j.1439-0329.2010.00646.x
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发表时间：
2011-04-01
期刊：
影响因子：
1.4
通讯作者：
Shiraishi, S.
中科院分区：
文献类型：
--
作者：
Nose, M.;Shiraishi, S.
日本黑松（Pinus thunbergii）极易感染由松材线虫（PWN; Bursaphelenchus xylophilus）引起的松材枯萎病。为了应对这种疾病，研究人员和树木育种者在以前的育种计划中选择了抗pwn的个体。为了了解日本黑松的抗性机制，我们建立了四个LongSAGE（基因表达序列分析）文库。共研究了20818个标记，其中5194个标记来自接种了PWN的抗性松，5218个标记来自未接种的抗性松，5194个标记来自接种了PWN的非抗性松，5212个标记来自未接种的非抗性松。文库分析表明，接种PWN后，14个标记种（发病相关蛋白2和4、渗透素、脂氧合酶和查尔酮合成酶）均显著上调，9个标记种（真核翻译起始因子SUI1、翻译控制肿瘤蛋白和木葡聚糖内转糖基化酶）均显著下调。另一方面，38个标记种仅在抗性松木中有较高水平的显著表达（过氧化氢酶、二烯内酯水解酶家族蛋白），25个标记种在非抗性松木中有较高水平的表达（致病相关蛋白1、2、3和白花青素双加氧酶）。这些差异表达的基因被认为反映了抗和非抗松树之间的一些差异。我们的研究结果为研究抗性和非抗性松树对PWN入侵的复杂反应提供了有价值的信息。
P>The Japanese black pine (Pinus thunbergii) is highly susceptible to pine wilt disease caused by the pine wood nematode (PWN; Bursaphelenchus xylophilus). To cope with this disease, researchers and tree breeders selected PWN-resistant individuals in a previous breeding program. In an attempt to understand the mechanisms of resistance in the Japanese black pine, we created four LongSAGE (serial analysis of gene expression) libraries. A total of 20 818 tags were studied, including 5194 tags from a PWN inoculated resistant pine, 5218 a non-inoculated resistant pine, 5194 an inoculated non-resistant pine, and 5212 a non-inoculated non-resistant pine. The analysis of the libraries indicated that 14 tag species were significantly up-regulated (e.g., pathogenesis-related proteins 2 and 4, osmotin, lipoxygenase, and chalcone synthase), and nine were down-regulated (eukaryotic translation initiation factor SUI1, translationally controlled tumor protein, and xyloglucan endotransglycosylase) by the PWN inoculation in both the resistant and non-resistant pines. On the other hand, 38 tag species were significantly expressed at a higher level only in the resistant pine (catalase, dienelactone hydrolase family protein) and 25 were expressed at a higher level in the non-resistant pine (pathogenesis-related proteins 1, 2, and 3, and leucoanthocyanidin dioxygenase). These differentially expressed genes are presumed to reflect some of the differences between the resistant and non-resistant pines. Our results provide valuable information on the complex responses induced in the resistant and non-resistant pine trees in response to PWN invasion.