Chemical methods to detect S-nitrosation.
Chemical methods to detect S-nitrosation.
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DOI:
10.1016/j.cbpa.2010.10.006
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发表时间:
2011-02
影响因子:
7.8
通讯作者:
Xian, Ming
中科院分区:
文献类型:
--
作者:
Wang, Hua;Xian, Ming
Nitric oxide (NO) is a cell-signaling molecule involved in a number of physiological and pathophysiological processes. Modification of cysteine residues by NO (or NO metabolites), i.e. S-nitrosation, changes the function of a broad spectrum of proteins. This reaction represents an important post-translational modification that transduces NO dependent signals. However, the detection and quantification of S-nitrosation in biological samples remains a challenge mainly because of the lability of S-nitrosation products: S-nitrosothiols (SNO). In this review we summarize recent developments of the methods to detect S-nitrosation. Our focus is the methods which can be used to directly conjugate the site(s) of S-nitrosation.
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