Metabolic and immunological effects of gut microbiota in leaf beetles at the local and systemic levels
Metabolic and immunological effects of gut microbiota in leaf beetles at the local and systemic levels
复制标题
叶甲虫肠道微生物群在局部和全身水平的代谢和免疫学影响
DOI：
10.1111/1749-4877.12528
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发表时间：
2021-03-25
影响因子：
3.3
通讯作者：
Xu, Letian
中科院分区：
文献类型：
--
作者：
Ma, Meiqi;Tu, Chengjie;Xu, Letian
昆虫肠道微生物对宿主的生理特性有着深远的影响，可以在局部（肠道）和全身（身体）水平上影响其生理。然而，宿主-微生物群相互作用的分子机制，特别是在非模式昆虫中，仍然难以捉摸。最近，组织特异性转录组学分析已被强调为研究宿主-微生物相互作用的强大工具。花柳蛾是一种世界性的食叶害虫，主要危害柳树和白杨。肠道微生物区系和这种宿主甲虫之间的相互作用还有待研究。在此，我们研究了肠道微生物群对杂色拟青霉幼虫体重的影响，比较了肠道微生物群存在和不存在的情况下幼虫的营养状况，并分析了整个发育幼虫阶段的肠道细菌负荷。然后，我们在无菌和非无菌幼虫的肠道和身体组织进行比较转录组学分析。最后，我们确定了营养代谢和免疫的代表基因的表达模式。结果表明，在常规幼虫中，体重生长被延迟，伴随着到第5（th）发育日的总细菌负荷的增加，并且无菌幼虫比常规饲养的幼虫具有更高的葡萄糖含量。营养和免疫分析表明，肠道细菌是甲虫幼虫发育的负担。这些发现阐明了肠道微生物群对P. versicolora的影响，并在遗传水平上深入了解了这种害虫对肠道微生物群的组织特异性反应，促进了我们对叶甲虫及其他宿主-微生物相互作用的分子机制的理解。
Insects' intestinal microbes have profound effects on the host's physiological traits, which can impact their physiology at both the local (gut) and systemic (body) levels. Nevertheless, the molecular mechanisms underlying host-microbiota interactions, especially in non-model insects, remain elusive. Recently, tissue-specific transcriptomic analysis has been highlighted as a robust tool in studying host-microbe interactions. Plagiodera versicolora is a worldwide leaf-eating pest that primarily feeds on willows and poplar. The interplay between gut microflora and this host beetle has yet to be studied. Herein, we investigate the effects of the gut microbiota on the body mass of P. versicolora larvae, compare the nutrition status of larvae in absence and presence of gut microbiota, and profile gut bacterial loads throughout its developmental larval stages. We then perform comparative transcriptomic analysis of gut and body tissues in axenic and non-axenic larvae. Finally, we confirm the expression patterns of representative genes in nutritional metabolism and immunity. Results show that weight growth is retarded in conventional larvae, with a concomitant increase of total bacterial load by the 5(th) development day, and germ-free larvae have a higher glucose content than conventional-reared larvae. Both nutritional and immunological analyses indicate that gut bacteria are a burden in the beetle's larval development. These findings elucidate the impacts of gut microbiota on P. versicolora, and provide insight into tissue-specific responses to gut microflora in this pest at the genetic level, boosting our understanding of the molecular mechanisms underlying host-microbe interactions in leaf beetles and beyond.