Two-photon imaging of lymphocyte motility and antigen response in intact lymph node
Two-photon imaging of lymphocyte motility and antigen response in intact lymph node
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DOI：
10.1126/science.1070051
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发表时间：
2002-06-07
期刊：
影响因子：
56.9
通讯作者：
Cahalan, MD
中科院分区：
文献类型：
--
作者：
Miller, MJ;Wei, SH;Cahalan, MD
淋巴细胞运动对于淋巴器官内的运输和与抗原呈递细胞的初始接触至关重要。这些过程的可视化以前仅限于体外系统。我们描述了使用双光子激光显微镜成像的动态行为的单个活淋巴细胞深在完整的淋巴结。在自然环境中，T细胞的峰值速度超过每分钟25微米，运动系数是B细胞的5到6倍。抗原挑战改变了T细胞从随机行走到“群”和稳定集群的轨迹。实时双光子成像揭示淋巴细胞的行为是基本的免疫反应的起始。
Lymphocyte motility is vital for trafficking within lymphoid organs and for initiating contact with antigen-presenting cells. Visualization of these processes has previously been, limited to in vitro systems. We describe the use of two-photon laser microscopy to image the dynamic behavior of individual living lymphocytes deep within intact lymph nodes. in their native environment, T cells achieved peak velocities of more than 25 micrometers per minute, displaying a motility coefficient that is five to six times that of B cells. Antigenic challenge changed T cell trajectories from random walks to "swarms" and stable clusters. Real-time two-photon imaging reveals lymphocyte behaviors that are fundamental to the initiation of the immune response.