Antibacterial effect of various shapes of silver nanoparticles monitored by SERS
Antibacterial effect of various shapes of silver nanoparticles monitored by SERS
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DOI：
10.1016/j.talanta.2015.02.022
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发表时间：
2015-06-01
期刊：
影响因子：
6.1
通讯作者：
Lendl, Bernhard
中科院分区：
文献类型：
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作者：
El-Zahry, Marwa R.;Mahmoud, Amer;Lendl, Bernhard
采用不同的方法对不同形状的银纳米粒子(AgNPs)的抗菌效果进行了比较评价。用化学方法制备了平均尺寸为40 nm的球形、三角形和六角形AgNPs，并用透射电子显微镜和紫外可见光谱对其进行了表征。通过表面增强拉曼光谱(SERS)、生长曲线和抑菌圈的测定，研究了不同AgNPs对革兰氏阴性杆菌的抑菌效果。SERS被证明是监测细菌细胞与AgNPs相互作用时发生的变化的灵敏方法，与参考方法提供的数据定性地进行了比较。然而，由于SERS已经对由于AgNPs的抗菌作用而导致的细菌化学的初始变化很敏感，SERS提供了快速和详细的信息，而不是基于生长曲线和抑制区评估的经典参考方法。本工作的结果还表明，与其他形状的纳米粒子相比，六角形的AgNPs显示出最高的抗菌效果，而三角形的AgNPs在所采用的条件下没有显示出抗菌效果。(C)2015爱思唯尔B.V.保留所有权利。
A comparative evaluation of antimicrobial effect of synthesized silver nanoparticles (AgNPs) of different shapes using different methods was performed. Spherical, triangular and hexagonal AgNPs with an average size of 40 nm were chemically prepared and characterized by transmission electron microscope (TEM) and UV-visible spectroscopy. The antimicrobial effect of these different AgNPs against the gram negative bacterium Escherichia coli (E. coil) was studied by surface enhanced Raman spectroscopy (SERS), the evaluation of growth curves and inhibition zones. SERS proved to be sensitive to monitor the changes that occurred in the bacterial cells upon interaction with AgNPs, which qualitatively compared well with the data provided by the reference methods. However, as SERS is already sensitive to initial changes in the chemistry of bacteria due to the antibacterial effect of the AgNPs, fast and detailed information is provided by SERS as opposed to the classical reference methods based on the evaluation of growth curves and inhibition zones. The results of this work also demonstrate that hexagonal AgNPs display the highest antibacterial effect when compared to other NPs shapes, with triangular AgNPs exhibiting no antibacterial effect under the adopted conditions. (C) 2015 Elsevier B.V. All rights reserved.