Alkaline Phosphatase-Instructed Self-Assembly of Gadolinium Nanofibers for Enhanced T2-Weighted Magnetic Resonance Imaging of Tumor
Alkaline Phosphatase-Instructed Self-Assembly of Gadolinium Nanofibers for Enhanced T2-Weighted Magnetic Resonance Imaging of Tumor
复制标题
碱性磷酸酶指导的钆纳米纤维自组装用于增强肿瘤 T2 加权磁共振成像
DOI：
10.1021/acs.analchem.7b00621
复制
发表时间：
2017-07-04
影响因子：
7.4
通讯作者：
Liang, Gaolin
中科院分区：
文献类型：
--
作者：
Dong, Ling;Qian, Junchao;Liang, Gaolin
Alkaline phosphatase (ALP) is an important enzyme but using ALP-instructed self-assembly of gadolinium nanofibers for enhanced T-2-weighted magnetic resonance imaging (MRI) of tumor has not been reported. In this work, we rationally designed a hydrogelator Nap-FFFYp-EDA-DOTA(Gd) (1P) which, under the catalysis of ALP, was able to self-assemble into gadolinium nanofibers to form hydrogel Gel I for enhanced T-2-weighted MR imaging of ALP activity in vitro and in tumor. T-2 phantom MR imaging indicated that the transverse relaxivity (r(2)) value of Gel I was 33.9% higher than that of 1P and both of them were 1 order of magnitude higher than that of Gd-DTPA. In vivo T-2-weighted MR imaging showed that, at 9.4 T, ALP-overexpressing HeLa tumors of 1P-injected mice showed obviously enhanced T-2 contrast. We anticipate that, by replacing ALP with other enzymes, our approach could be applied for MR diagnosis of other diseases in the future.
