Expression of aquaporins 1 and 5 in a model of ventilator-induced lung injury and its relation to tidal volume
Expression of aquaporins 1 and 5 in a model of ventilator-induced lung injury and its relation to tidal volume
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DOI：
10.1113/ep085729
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发表时间：
2016-11-01
影响因子：
2.7
通讯作者：
Javier Belda, Francisco
中科院分区：
文献类型：
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作者：
Fabregat, Gustavo;Garcia-de-la-Asuncion, Jose;Javier Belda, Francisco
我们分析了呼吸机诱导的肺损伤模型中水通道蛋白1和5的表达及其与潮气量的关系。使用了42只大鼠。处死6只非通气组动物(对照组)。其余大鼠以不同潮气量(7ml7mlkg(-1)和20mlkg20mlkg(-1))换气2小时，呼吸频率为90次/分。用免疫印迹法检测肺泡灌洗液中AQP1、AQP5的表达，并测定肺组织中AQP5的表达。20ml组大鼠肺组织水肿和肺泡毛细血管膜通透性明显高于对照组。与对照组相比，7ml和20ml组AQP1的表达明显降低。综上所述，高潮气量机械通气会引起肺损伤和水肿，增加肺通透性。高潮气量通气组大鼠肺组织中AQP1的表达减少。
We analysed the expression of aquaporins 1 and 5 and its relation with tidal volume in a model of ventilator-induced lung injury. Forty-two rats were used. Six non-ventilated animals were killed (control group). The remaining rats were ventilated for 2h with different tidal volumes (group 7ML with 7mlkg(-1) and group 20ML with 20mlkg(-1)) and a respiratory rate of 90breathsmin(-1). Lung oedema was measured, and the expression of AQP1 and AQP5 was determined by Western immunoblotting and measurement of mRNA. Lung oedema and alveolar-capillary membrane permeability were significantly increased in the animals of group 20ML compared with the control group. Expression of AQP1 was decreased in groups 7ML and 20ML compared with the control group. In conclusion, mechanical ventilation with a high tidal volume causes lung injury and oedema, increasing lung permeability. In rats ventilated with a high tidal volume, the pulmonary expression of AQP1 decreases.