Observation of antibacterial effect of biodegradable polymeric nanoparticles on Staphylococcus epidermidis biofilm using FE-SEM with an ionic liquid.
Observation of antibacterial effect of biodegradable polymeric nanoparticles on Staphylococcus epidermidis biofilm using FE-SEM with an ionic liquid.
复制标题
使用离子液体的 FE-SEM 观察可生物降解的聚合物纳米粒子对表皮葡萄球菌生物膜的抗菌效果。
作者：
Takahashi;C.;Ogawa;N.;Kawashima;Y.;Yamamoto;H.
利用场发射扫描电子显微镜（FE-SEM）观察了可生物降解的纳米聚合物（NPs）对表皮葡萄球菌（Staphylococcus epidermidisis）生物膜的抗菌作用。使用FE-SEM观察亲水性离子液体1-丁基-3-甲基咪唑四氟硼酸盐（[BMIM][BF 4]）。利用该方法和共聚焦激光扫描显微镜揭示了三种类型的纳米颗粒在粘附和渗透行为上的差异。采用乳化溶剂扩散法制备了可生物降解的聚丙交酯-乙交酯（PLGA）纳米粒。在这项研究中，我们用三种PLGA纳米颗粒处理生物膜：未修饰的PLGA，用壳聚糖（CS）修饰的PLGA和克拉霉素（CAM）负载+CS修饰的PLGA。S.使用LIVE/DEAD BacLight试剂盒估计用PLGA NP处理的表皮细胞的抗菌能力，以定量方式了解每个NP样品的抗菌能力。结果表明，CAM负载+CS修饰的PLGA对生物膜具有较高的抗菌能力。这种新的观察技术将是有用的药物制剂和医疗代理的发展。
We successfully visualized the antibacterial behavior of biodegradable polymeric nanoparticles (NPs) on a biofilm formed byStaphylococcus epidermidisusing field emission scanning electron microscopy (FE-SEM). A hydrophilic ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]), was applied for observation using FE-SEM. The differences in adherence and penetration behavior of three types of NPs were revealed using this method and confocal laser scanning microscopy. Biodegradable poly(dl-lactide-co-glycolide) (PLGA) NPs were prepared by the emulsion solvent diffusion method. In this study, we treated the biofilm with three PLGA NPs: unmodified PLGA, PLGA modified with chitosan (CS) and clarithromycin (CAM)-loaded + CS-modified PLGA. The viability ofS. epidermidiscells treated with PLGA NPs was estimated using the LIVE/DEAD BacLight kit to understand the antibacterial ability of each NP sample in a quantitative way. The results confirmed that CAM-loaded + CS-modified PLGA had high antibacterial ability on the biofilm. This novel observation technique would be useful in the development of drug formations and medical agents.