The predominant Actinomyces spp. isolated from infected dentin of active root caries lesions
The predominant Actinomyces spp. isolated from infected dentin of active root caries lesions
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DOI：
10.1177/00220345990780090701
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发表时间：
1999-09-01
影响因子：
7.6
通讯作者：
Beighton, D
中科院分区：
文献类型：
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作者：
Brailsford, SR;Tregaskis, RB;Beighton, D
放线菌是革兰氏阳性多形性杆状菌（GPPR），在所有哺乳动物的口腔微生物群中占很大比例。尽管它们在龋齿发生和进展中的作用尚不清楚，但它们与根龋有关。许多研究都集中于内氏放线菌，但很少有报告记录 GPPR 的其他成员。因此，我们研究了从活动性根龋病变的受感染牙本质中分离出的 GPPR（n = 9），以确定最常分离到的物种。 GPPR 在需氧和厌氧条件下进行分离，并根据新的分类法通过生化和生理测试鉴定到物种水平。在调查的 654 个 GPPR 分离株中，有 607 个被鉴定为属于放线菌属。其中，242 株被鉴定为 A. israelii，225 株为 A. gerencseriae，109 株为 A. naeslundii，15 株为 A. odontolyticus，13 株为 A. georgiae。还通过重复外基因回文聚合酶链反应 (REP-PCR) 在 DNA 水平上研究了 A. israelii (n = 56) 和 A. gerencseriae (n = 46) 的各个菌株，以研究克隆多样性。尽管仅研究了少量分离株，但 REP-PCR 显示，A. gerencseriae 和 A. israelii 群体的基因型在各个根龋病灶内是异质的。来自相同病变的 A. gerencseriae 和 A. israelii 菌株不具有相同的 REP-PCR 模式，显示了鉴定方案的稳健性。从需氧平板中分离出的 A. gerencseriae 比例显着更高 (p < 0.05)，而从厌氧平板中分离出的 A. israelii 比例显着更高 (p < 0.05)。单个放线菌的作用。根龋过程中的作用尚不清楚，因为不同群体的 GPPR 是从各个活动性根龋病变中分离出来的。
Actinomyces are Gram-positive pleomorphic rods (GPPR) which form a large proportion of the oral microflora of all mammals. They have been implicated in root caries, although their role in dental caries initiation and progression is not well-understood. Many studies have focused on Actinomyces naeslundii, but few reports have documented other members of the GPPR. Therefore, we investigated the GPPRs isolated from infected dentin of active root caries lesions (n = 9) to determine which species were the most frequently isolated. The GPPR were isolated under both aerobic and anaerobic conditions and identified by biochemical and physiological tests to the species level according to the new taxonomy. Of 654 GPPR isolates investigated, 607 were identified as belonging to the genus Actinomyces. Of these, 242 were identified as A. israelii, 225 as A. gerencseriae, 109 as A. naeslundii, 15 as A. odontolyticus, and 13 as A. georgiae. Individual strains of A. israelii (n = 56) and A. gerencseriae (n = 46) were also investigated at the DNA level by means of Repetitive Extragenic Palindromic polymerase chain-reactions (REP-PCR) for the study of clonal diversity. Although only a small number of isolates was investigated, REP-PCR showed that the genotypes of both A. gerencseriae and A. israelii populations were heterogeneous within individual root caries lesions. A. gerencseriae and A. israelii strains from the same lesions did not share the same REP-PCR patterns, showing the robustness of the identification scheme. A significantly greater proportion of A. gerencseriae was isolated from the aerobic plates (p < 0.05), while the proportion of A. israelii was significantly (p < 0.05) greater from anaerobic plates. The role of individual Actinomyces spp. in the root caries process remains unclear, since various populations of GPPRs were isolated from individual active root caries lesions.